Abstract |
The presence of tumor necrosis factor-alpha ( TNF-alpha) during endotoxemia in ruminants has not been reported previously. In this study, we detected the in vivo release of bovine TNF-alpha by using WEHI-164 murine fibrosarcoma cells as targets in an 18-h cytotoxicity assay. Treatment of the WEHI-164 cells with 1 microgram of actinomycin D ( dactinomycin) enhanced approximately twofold the susceptibility of the cells to TNF-alpha activity. TNF-alpha activity in sera from neonatal calves injected intravenously with 2.7 micrograms of Escherichia coli lipopolysaccharide (LPS) increased rapidly within the first 2 h postinjection and then declined until it was undetectable by 4 h postinjection. Sera taken before LPS administration had no TNF-alpha activity. LPS (10 micrograms/ml) and fetal, newborn, and pooled adult bovine sera alone and in combination had no direct cytotoxic effects on WEHI-164 cells. TNF-alpha cytotoxic activity is probably not due to the presence of interleukin-1 (IL-1), alpha interferon, or gamma interferon in the sera since recombinant human IL-1, natural bovine IL-1, and recombinant bovine alpha and gamma interferons had no direct cytotoxic effects on WEHI-164 cells. A monoclonal antibody that neutralizes recombinant human TNF-alpha significantly reduced the cytotoxic activity of sera from LPS-injected calves.
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Authors | J L Adams, S D Semrad, C J Czuprynski |
Journal | Journal of clinical microbiology
(J Clin Microbiol)
Vol. 28
Issue 5
Pg. 998-1001
(May 1990)
ISSN: 0095-1137 [Print] United States |
PMID | 2112564
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S., Research Support, U.S. Gov't, P.H.S.)
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Chemical References |
- Endotoxins
- Interferon Type I
- Interleukin-1
- Lipopolysaccharides
- Tumor Necrosis Factor-alpha
- Interferon-gamma
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Topics |
- Animals
- Animals, Newborn
- Cattle
- Cytotoxicity Tests, Immunologic
- Endotoxins
(toxicity)
- Interferon Type I
(pharmacology)
- Interferon-gamma
(pharmacology)
- Interleukin-1
(pharmacology)
- Lipopolysaccharides
(toxicity)
- Toxemia
(blood, etiology)
- Tumor Cells, Cultured
- Tumor Necrosis Factor-alpha
(metabolism)
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