Abstract | OBJECTIVE: METHODS: the survivin ASODN was designed and synthesized and then respectively transfected into Mc3 cells. The morphological changes of the Mc3 cells were observed 24, 48 and 72 h after transfection by inverted microscope and the apoptosis rate detected by flow cytometry. Methyl thiazolyl tetrazolium (MTT) assay was used to detect the effect of the transfection on cell poliferation, terminal deoxynucleotidyl transferase-mediated dUTP- biotin nick end labeling (TUNEL) method for analysis of apoptotic index, and semi-quantitative reverse transcriptase polymerase chain reaction (RT-PCR) to detect the expression of survivin. RESULTS: in survivin ASODN transfection group, there was less Mc3 cells than in other groups. The suspended cells dropping from the wall increased and showed typical apoptotic changes and time-dependent. Mc3 cell apoptosis rate in survivin ASODN transfection group transfected for 24, 48, 72 h was 12.96%, 14.43%, 22.69%, respectively, which were significantly higher than in other groups (P < 0.01) and time-dependent (P < 0.05). The inhibitory rate of Mc3 cells in survivin ASODN transfection group was 22.35%, 39.04%, 43.46%, which were significantly higher than other groups (P < 0.01) and time-dependent (P < 0.05). The apoptosis index (AI) of Mc3 cells in survivin ASODN transfection group was 11.038%, 12.172%, 18.900%, significantly higher than other groups (P < 0.01) and time-dependent (P < 0.05). The survivin mRNA levels in Mc3 cells were 0.739 ± 0.008, 0.668 ± 0.007, 0.500 ± 0.006, and the relative inhibition rate in these cells was 18.21%, 26.06%, 44.82%, significantly lower than other groups (P < 0.01) and time-dependent manner (P < 0.01). CONCLUSIONS:
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Authors | Hong Qi, Jian Guo, Yin-Cheng Zahng, Shu-Wei Li, Li-Lin Yang |
Journal | Zhonghua kou qiang yi xue za zhi = Zhonghua kouqiang yixue zazhi = Chinese journal of stomatology
(Zhonghua Kou Qiang Yi Xue Za Zhi)
Vol. 45
Issue 9
Pg. 525-30
(Sep 2010)
ISSN: 1002-0098 [Print] China |
PMID | 21122444
(Publication Type: Journal Article)
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Chemical References |
- BIRC5 protein, human
- Inhibitor of Apoptosis Proteins
- Microtubule-Associated Proteins
- Neoplasm Proteins
- Oligonucleotides, Antisense
- RNA, Messenger
- Survivin
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Topics |
- Apoptosis
- Carcinoma, Mucoepidermoid
(metabolism)
- Cell Line, Tumor
- Cell Proliferation
- Gene Targeting
- Humans
- Inhibitor of Apoptosis Proteins
(genetics)
- Microtubule-Associated Proteins
- Neoplasm Proteins
- Oligonucleotides, Antisense
- RNA, Messenger
- Salivary Gland Neoplasms
(metabolism)
- Survivin
- Transfection
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