Different protein profile in amniotic fluid with nervous system malformations by surface-enhanced laser desorption-ionization/time-of-flight mass spectrometry (SELDI-TOF-MS) technology.

Abstract

AIM:

To detect the distinct proteins in amniotic fluid (AF) between nervous system malformations fetuses and normal fetuses.

MATERIAL AND METHODS:

Surface-enhanced laser desorption-ionization/time-of-flight mass spectrometry was used to characterize AF peptides in AF between nervous system malformations fetuses and normal fetuses. WCX2 protein chips were used to characterize AF peptides in AF. Protein chips were examined in a PBSIIC protein reader, the protein profiling was collected by ProteinChip software version 3.1 (Ciphergen Biosystems, Fremont, CA, USA) and analyzed by Biomarker Wizard software (Ciphergen Biosystems). Nine distinct proteins were identified in AF between nervous system malformations fetuses and normal fetuses.

RESULTS:

Compared with the control group, three proteins with m/z 4967.5 Da, 5258.0 Da, and 11,717.0 Da were down-regulated, and six proteins with m/z 2540.4 Da, 3107.1 Da, 3396.8 Da, 4590.965 Da, 5589.2 Da and 6429.4 Da up-regulated in nervous system malformations fetuses.

CONCLUSION:

The results suggest that there are distinct proteins in protein profiling of AF between nervous system malformations fetuses and normal fetuses.

• Authors: Zhe Ma, Cun Liu, Biping Deng, Shaogang Dong, Guowei Tao, Xinfeng Zhan, Chuner Wang, Shaoping Liu, Xun Qu
• Journal: The journal of obstetrics and gynaecology research (J Obstet Gynaecol Res) Vol. 36 Issue 6 Pg. 1195-203 (Dec 2010) ISSN: 1447-0756 [Electronic] Australia
• PMID: 21114572 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © 2010 The Authors. Journal of Obstetrics and Gynaecology Research © 2010 Japan Society of Obstetrics and Gynecology.

Chemical References

• Biomarkers

Topics

• Adult
• Amniotic Fluid (metabolism)
• Biomarkers (metabolism)
• Databases, Protein
• Female
• Fetal Diseases (diagnosis, metabolism)
• Humans
• Nervous System Malformations (diagnosis, metabolism)
• Peptide Mapping
• Pregnancy
• Prenatal Diagnosis
• Reproducibility of Results
• Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
• Young Adult