Contributions of steroidogenic factor 1 to the transcription landscape of Y1 mouse adrenocortical tumor cells.

Abstract

The contribution of steroidogenic factor 1 (SF-1) to the gene expression profile of Y1 mouse adrenocortical cells was evaluated using short hairpin RNAs to knockdown SF-1. The reduced level of SF-1 RNA was associated with global changes that affected the accumulation of more than 2000 transcripts. Among the down-regulated transcripts were several with functions in steroidogenesis that were affected to different degrees--i.e., Mc2r>Scarb1>Star≥Hsd3b1>Cyp11b1. For Star and Cyp11b1, the different levels of expression correlated with the amount of residual SF-1 bound to the proximal promoter regions. The knockdown of SF-1 did not affect the accumulation of Cyp11a1 transcripts even though the amount of SF-1 bound to the proximal promoter of the gene was reduced to background levels. Our results indicate that transcripts with functions in steroidogenesis vary in their dependence on SF-1 for constitutive expression. On a more global scale, SF-1 knockdown affects the accumulation of a large number of transcripts, most of which are not recognizably involved in steroid hormone biosynthesis.

• Authors: Bernard P Schimmer, Jennivine Tsao, Martha Cordova, Sara Mostafavi, Quaid Morris, Joshua O Scheys
• Journal: Molecular and cellular endocrinology (Mol Cell Endocrinol) Vol. 336 Issue 1-2 Pg. 85-91 (Apr 10 2011) ISSN: 1872-8057 [Electronic] Ireland
• PMID: 21111771 (Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)
• Copyright: Crown Copyright © 2010. Published by Elsevier Ireland Ltd. All rights reserved.

Chemical References

• Phosphoproteins
• RNA, Messenger
• RNA, Small Interfering
• Steroidogenic Factor 1
• Steroids
• steroidogenic acute regulatory protein
• steroidogenic factor 1, mouse
• Steroid 11-beta-Hydroxylase
• Cholesterol Side-Chain Cleavage Enzyme

Topics

• Adrenal Cortex Neoplasms (genetics)
• Animals
• Cell Line, Tumor
• Cholesterol Side-Chain Cleavage Enzyme (genetics)
• Chromatin Immunoprecipitation
• Clone Cells
• Gene Expression Profiling
• Gene Expression Regulation, Neoplastic
• Gene Knockdown Techniques
• Mice
• Phenotype
• Phosphoproteins (genetics, metabolism)
• Promoter Regions, Genetic (genetics)
• Protein Binding
• RNA, Messenger (genetics, metabolism)
• RNA, Small Interfering (metabolism)
• Steroid 11-beta-Hydroxylase (genetics)
• Steroidogenic Factor 1 (genetics, metabolism)
• Steroids (biosynthesis)
• Transcription, Genetic
• Transformation, Genetic