Silencing Id-1 inhibits lymphangiogenesis through down-regulation of VEGF-C in oral squamous cell carcinoma.

Abstract

Our previous study demonstrated that overexpression of Id-1 (inhibitor of differentiation/DNA binding) was associated with lymphatic metastasis in human oral squamous cell carcinoma (OSCC). In this study, we further unveiled the association of Id-1 with vascular endothelial growth factor-C (VEGF-C) and peritumoral lymphatic vessel density (PLVD), and the effect of silencing Id-1 on inhibiting lymphangiogenesis in OSCC. We found that Id-1 was associated with VEGF-C (r=0.569, p<0.001) and PLVD (r=0.240, p<0.001) in OSCC. Lentivirus-mediated RNA interference targeting Id-1 in an OSCC cell line Tca8113 resulted in down-regulation of VEGF-C (p=0.003, 0.007). Moreover, when Id-1 was suppressed by injecting Id-1-siRNA-lentivirus into the transplanted tumors in nude mice, VEGF-C was down-regulated (p=0.018) and the PLVD decreased (p=0.001). Our results suggest that Id-1 was correlated with lymphangiogenesis in OSCC. Silencing Id-1 could inhibit lymphangiogenesis through down-regulation of VEGF-C and it might be a promising treatment modality for the lymphatic metastasis of OSCC.

• Authors: Zuoqing Dong, Fengcai Wei, Chengjun Zhou, Tomoki Sumida, Hiroyuki Hamakawa, Yingwei Hu, Shaohua Liu
• Journal: Oral oncology (Oral Oncol) Vol. 47 Issue 1 Pg. 27-32 (Jan 2011) ISSN: 1879-0593 [Electronic] England
• PMID: 21111670 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2010 Elsevier Ltd. All rights reserved.

Chemical References

• ID1 protein, human
• Inhibitor of Differentiation Protein 1
• Vascular Endothelial Growth Factor C

Topics

• Animals
• Carcinoma, Squamous Cell (genetics, metabolism)
• Cell Line, Tumor
• Down-Regulation
• Gene Silencing (physiology)
• Humans
• Inhibitor of Differentiation Protein 1 (genetics, metabolism)
• Lymphangiogenesis (genetics)
• Lymphatic Metastasis
• Mice
• Mice, Nude
• Mouth Neoplasms (genetics, metabolism)
• Vascular Endothelial Growth Factor C (genetics, metabolism)