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Results of a European interlaboratory method validation study for the quantitative determination of lipophilic marine biotoxins in raw and cooked shellfish based on high-performance liquid chromatography-tandem mass spectrometry. Part I: collaborative study.

Abstract
A European interlaboratory collaborative study was conducted to validate a method for the quantitative determination of lipophilic marine biotoxins based on high-performance liquid chromatography-tandem mass spectrometry. During this study, the diarrhetic shellfish poisoning toxins okadaic acid, dinophysis toxin1 and 2 including their esters, the azaspiracids 1-3, pectenotoxin2, and the yessotoxins were investigated at concentration levels near the limit of quantification and near the legal limit. Naturally contaminated blue mussels, both raw and cooked and spiked extracts of clams and oysters were studied and results were obtained for 16 test samples from 16 laboratories representing eight different countries. This article summarizes the study outcome concerning validation key parameters like specificity, linearity, limit of detection, accuracy/recovery, and precision. Further, influences of cooking of mussels before homogenization or hydrolysis on method robustness have been evaluated.
AuthorsAnja These, Christine Klemm, Ingo Nausch, Steffen Uhlig
JournalAnalytical and bioanalytical chemistry (Anal Bioanal Chem) Vol. 399 Issue 3 Pg. 1245-56 (Jan 2011) ISSN: 1618-2650 [Electronic] Germany
PMID21107979 (Publication Type: Journal Article, Multicenter Study, Validation Study)
Chemical References
  • Marine Toxins
  • Okadaic Acid
Topics
  • Animals
  • Chromatography, High Pressure Liquid
  • Cooking
  • Europe
  • Food Analysis (methods)
  • International Cooperation
  • Marine Toxins (analysis)
  • Okadaic Acid (analysis)
  • Reproducibility of Results
  • Sensitivity and Specificity
  • Shellfish (analysis)
  • Tandem Mass Spectrometry

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