Five-minute Giemsa stain for rapid detection of malaria parasites in blood smears.

Abstract

The Giemsa stain is used as the gold standard for the diagnosis of malaria on blood smears. The classical staining procedure requires between 30 and 45 min. We modified the Giemsa stain and reduced the staining time to 5 min without any loss of quality.

• Authors: M M Jager, J L Murk, R D Piqué, T A M Hekker, C M J E Vandenbroucke-Grauls
• Journal: Tropical doctor (Trop Doct) Vol. 41 Issue 1 Pg. 33-5 (Jan 2011) ISSN: 1758-1133 [Electronic] England
• PMID: 21088023 (Publication Type: Evaluation Study, Journal Article)

Chemical References

• Azure Stains
• Coloring Agents

Topics

• Animals
• Azure Stains
• Blood Specimen Collection (methods)
• Coloring Agents
• Humans
• Malaria (blood, diagnosis, parasitology)
• Parasitemia (blood, diagnosis, parasitology)
• Plasmodium falciparum (isolation & purification)
• Plasmodium ovale (isolation & purification)
• Plasmodium vivax (isolation & purification)
• Sensitivity and Specificity
• Staining and Labeling (methods)
• Time Factors