Immunoexpression of bone morphogenetic protein-2 (BMP-2), BMP receptor type IA, and BMP receptor type II in metastatic and non-metastatic lower lip squamous cell carcinoma.

Abstract

BACKGROUND:

The aim of this study was to evaluate the expression of bone morphogenetic protein-2 (BMP-2), BMPR-IA, and BMPR-II in metastatic and non-metastatic lower lip squamous cell carcinoma (LLSCC).

METHODS:

Twenty LLSCC with regional nodal metastasis and 20 LLSCC without metastasis were selected for analysis of the immunoexpression of BMP-2, BMPR-IA, and BMPR-II. The percentage of positive cells was evaluated according to the following parameters: score 1 (staining in 0-50% of cells), score 2 (staining in 51-75% of cells), and score 3 (staining in >75% of cells). Immunostaining intensity was evaluated according to the following scores: score 1 (absent or weak expression) and score 2 (strong expression).

RESULTS:

Regarding the percentage of positive cells, most tumors with and without metastasis showed score 3 for both BMP-2 (P = 0.655) and BMPR-IA (P = 0.655). For BMPR-II, metastatic tumors exhibited higher percentage of positive cells in comparison with non-metastatic tumors (P = 0.049). With respect to immunostaining intensity, most LLSCCs with and without metastasis showed score 2 for BMP-2 (P = 1.000) and score 1 for BMPR-II (P = 1.000). For BMPR-IA, most metastatic tumors presented score 2, whereas most non-metastatic tumors showed score 1 (P < 0.001). Strong expression of BMPR-IA showed a statistically significant association with advanced clinical staging (P < 0.001) and high score of malignancy (P = 0.028).

CONCLUSIONS:

The results of this study suggest that disturbances in the BMP-2 signaling pathway may be involved in the development of LLSCC and that the strong expression of BMPR-IA might be indicative of the development of metastasis in these lesions.

• Authors: Cyntia H P de Carvalho, Cassiano F W Nonaka, Cristina R F de Araújo, Lélia B de Souza, Leão P Pinto
• Journal: Journal of oral pathology & medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology (J Oral Pathol Med) Vol. 40 Issue 2 Pg. 181-6 (Feb 2011) ISSN: 1600-0714 [Electronic] Denmark
• PMID: 21059107 (Publication Type: Journal Article)
• Copyright: © 2010 John Wiley & Sons A/S.

Chemical References

• Bone Morphogenetic Protein 2
• Bone Morphogenetic Protein Receptors, Type I
• Bone Morphogenetic Protein Receptors, Type II

Topics

• Bone Morphogenetic Protein 2 (biosynthesis)
• Bone Morphogenetic Protein Receptors, Type I (biosynthesis)
• Bone Morphogenetic Protein Receptors, Type II (biosynthesis)
• Carcinoma, Squamous Cell (metabolism, pathology, secondary)
• Humans
• Immunoenzyme Techniques
• Lip Neoplasms (metabolism, pathology, secondary)
• Lymphatic Metastasis
• Signal Transduction
• Statistics, Nonparametric