Abstract | BACKGROUND AND AIM: METHODS: The stable hSSTR2-expressing A549 cells (pcDNA3-hSSTR2 A549) and non- somatostatin receptor expressing A549 cells (pcDNA3 A549) were selected by western blot. Later, a corresponding animal tumor model was established. Expression of the hSSTR2 reporter was imaged using (188)Re-RC-160 recognition. Tumors were evaluated for somatostatin receptor expression using immunohistochemistry. The distribution of (188)Re-RC-160 in the animal tumor model was measured and the inhibitory effects of (188)Re-RC-160 were evaluated by measurement of tumor growth and hematoxylin and eosin and TdT mediated dUTP nick end labeling (TUNEL) staining. RESULTS: In vivo radioimaging revealed specific targeting of (188)Re-RC-160 to tumors derived from pcDNA3- hSSTR2 A549 cells, compared to those from pcDNA3 A549 cells. pcDNA3- hSSTR2 A549 tumor growth inhibition was significantly higher in the single 7.4 MBq (188)Re-RC-160 treatment group than in the 2×7.4 MBq rhenium-188, RC-160 group, control group, and pcDNA3 A549 tumors (P<.05). Furthermore, treatment fractionation group (2 × 7.4 MBq (188)Re- RC-160), induced significantly increased tumor-growth inhibition compare with single 7.4 MBq (188)Re-RC-160 treatment (P<.05). CONCLUSION: These studies showed that (188)Re-RC-160 could be effectively used for targeting therapy the A549-derived tumors exogenously expressing hSSTR2, which will offers a potential therapeutic strategy for the treatment of somatostatin receptor-negative cancers.
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Authors | Rong Zhao, Weidong Yang, Zhe Wang, Guoquan Li, Weiwei Qin, Jing Wang |
Journal | Nuclear medicine and biology
(Nucl Med Biol)
Vol. 37
Issue 8
Pg. 977-87
(Nov 2010)
ISSN: 1872-9614 [Electronic] United States |
PMID | 21055629
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2010 Elsevier Inc. All rights reserved. |
Chemical References |
- Radioisotopes
- Receptors, Somatostatin
- vapreotide
- Somatostatin
- Rhenium
- somatostatin receptor 2
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Topics |
- Adenocarcinoma
(genetics, metabolism, pathology, radiotherapy)
- Adenocarcinoma of Lung
- Animals
- Blotting, Western
- Cell Line, Tumor
- Cell Transformation, Neoplastic
- Cloning, Molecular
- Humans
- Immunohistochemistry
- In Situ Nick-End Labeling
- Lung Neoplasms
(genetics, metabolism, pathology, radiotherapy)
- Male
- Mice
- Mice, Inbred BALB C
- Radioisotopes
(therapeutic use)
- Receptors, Somatostatin
(genetics, metabolism)
- Rhenium
(therapeutic use)
- Somatostatin
(analogs & derivatives, metabolism, pharmacokinetics, therapeutic use)
- Tomography, Emission-Computed, Single-Photon
- Transfection
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