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In vivo infection by Trypanosoma cruzi: the conserved FLY domain of the gp85/trans-sialidase family potentiates host infection.

Abstract
Trypanosoma cruzi is a protozoan parasite that infects vertebrates, causing in humans a pathological condition known as Chagas' disease. The infection of host cells by T. cruzi involves a vast collection of molecules, including a family of 85 kDa GPI-anchored glycoproteins belonging to the gp85/trans-sialidase superfamily, which contains a conserved cell-binding sequence (VTVXNVFLYNR) known as FLY, for short. Herein, it is shown that BALB/c mice administered with a single dose (1 μg/animal, intraperitoneally) of FLY-synthetic peptide are more susceptible to infection by T. cruzi, with increased systemic parasitaemia (2-fold) and mortality. Higher tissue parasitism was observed in bladder (7·6-fold), heart (3-fold) and small intestine (3·6-fold). Moreover, an intense inflammatory response and increment of CD4+ T cells (1·7-fold) were detected in the heart of FLY-primed and infected animals, with a 5-fold relative increase of CD4+CD25+FoxP3+ T (Treg) cells. Mice treated with anti-CD25 antibodies prior to infection, showed a decrease in parasitaemia in the FLY model employed. In conclusion, the results suggest that FLY facilitates in vivo infection by T. cruzi and concurs with other factors to improve parasite survival to such an extent that might influence the progression of pathology in Chagas' disease.
AuthorsR R Tonelli, A C Torrecilhas, J F Jacysyn, M A Juliano, W Colli, M J M Alves
JournalParasitology (Parasitology) Vol. 138 Issue 4 Pg. 481-92 (Apr 2011) ISSN: 1469-8161 [Electronic] England
PMID21040619 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Forkhead Transcription Factors
  • Foxp3 protein, mouse
  • Glycoproteins
  • Interleukin-2 Receptor alpha Subunit
  • Peptides
  • trans-sialidase
  • Neuraminidase
Topics
  • Amino Acid Sequence
  • Animals
  • CD4-Positive T-Lymphocytes (immunology)
  • Chagas Disease (immunology, parasitology)
  • Conserved Sequence
  • Female
  • Forkhead Transcription Factors (immunology)
  • Glycoproteins (chemistry, immunology)
  • Humans
  • Interleukin-2 Receptor alpha Subunit (immunology)
  • Mice
  • Mice, Inbred BALB C
  • Neuraminidase (chemistry, immunology)
  • Parasitemia (immunology, parasitology)
  • Peptides (administration & dosage, chemical synthesis, chemistry)
  • Peritoneum (cytology)
  • T-Lymphocytes, Regulatory (immunology)
  • Trypanosoma cruzi (enzymology, immunology, pathogenicity)
  • Virulence

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