[The study about expression of Gli2 in vein grafts].

Abstract

OBJECTIVE:

To study the expression of cell cycle related factor Gli2 in autogenous vein graft and its relation with neointima formation.

METHOD:

Autogenous vein graft model were established in 36 male wistar rats of 8 weeks old, 140 g, by transplanting the left jugular vein to intra renal abdominal aorta with microsurgical technique. Graft veins were harvest at 14, 28 days after transplantation. The IF and W-B were used to detect the protein expression in the vein graft. At the same time Gli2- mRNA was measured by RT-PCR.

RESULTS:

Immunofluorescent staining showed that the Gli2+ cells was only 3.2% ± 0.4% in the normal vein, but was much more in the vein graft after surgery, was 41.3% ± 0.6%, 58.3 ± 0.6% respectively; The expression of Gli2 and PCNA were both elevated in the vein graft. There is a positive correlation between them which indicated by W-B, the relation index was 0.826; the Gli2 mRNA content was also increased in vein graft, was 8.9, 13.6 fold compared with normal vein as 1 respectively.

CONCLUSION:

Gli2 is upregulated in autogenous vein grafts and may correlated with the proliferation of vascular smooth muscle cells.

• Authors: Feng-he Li, Shi-jie Xin, Dong Yang, Ze-shi Cui, Yu Yang, Jian Zhang, Zhi-quan Duan
• Journal: Zhonghua yi xue za zhi (Zhonghua Yi Xue Za Zhi) Vol. 90 Issue 29 Pg. 2078-81 (Aug 03 2010) ISSN: 0376-2491 [Print] China
• PMID: 21029651 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Kruppel-Like Transcription Factors
• Zinc Finger Protein Gli2

Topics

• Animals
• Kruppel-Like Transcription Factors (metabolism)
• Male
• Rats
• Rats, Wistar
• Transplantation, Autologous
• Tunica Intima (metabolism, pathology)
• Veins (metabolism, transplantation)
• Zinc Finger Protein Gli2