Abstract |
The diagnosis of dengue is an imperfect science. By 1983 we had sensitive isolation systems including arthropods and arthropod cell cultures, specific-and group-reactive monoclonal antibodies, and a rapid and sensitive immunoassay for IgM and IgG. Progress in the past 7 years encompasses the use of monocyte lines for primary isolation, a more rapid plaque reduction neutralization test with BHK-21 cells, labeled RNA probes to detect dengue-specific nucleic acid, and improved ELISA technology with specific synthetic and engineered antigens.
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Authors | R E Shope |
Journal | The Southeast Asian journal of tropical medicine and public health
(Southeast Asian J Trop Med Public Health)
Vol. 21
Issue 4
Pg. 642-5
(Dec 1990)
ISSN: 0125-1562 [Print] Thailand |
PMID | 2098929
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S., Research Support, U.S. Gov't, P.H.S., Review)
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Chemical References |
- Antibodies, Viral
- Antigens, Viral
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Topics |
- Antibodies, Viral
(analysis)
- Antigens, Viral
(analysis)
- Dengue
(diagnosis, epidemiology, immunology)
- Humans
- Immunoassay
(methods, trends)
- Sensitivity and Specificity
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