Anti-inflammatory and immunomodulatory effects of RDV-8 [C18H22N2O2S (ethyl 1-butyl-6-methyl-2-phenyl-4-thioxo-1,4-dihydropyrimidine-5-carboxylate)] in a rat model of induced pleurisy and in vitro lymphoproliferation.

Abstract

RDV-8 [C(18)H(22)N(2)O(2)S (ethyl 1-butyl-6-methyl-2-phenyl-4-thioxo-1,4-dihydropyrimidine-5-carboxylate)] is derived from the 4-thioxopyrimidine, and presents important clinical effects. The present study explored the RDV-8 effects in the proliferation of human peripheral blood mononuclear cells (PBMCs), as well as in a pleurisy-induced rat model. PBMCs were directly plated in four different RDV-8 concentrations (0.0125, 0.025, 0.05 and 0.1 mg/mL). RDV-8 decreased cell proliferation and monocyte chemotactic protein 1 synthesis. The interleukin 1 levels and the cytotoxic effect were not significantly affected by RDV-8 treatment. In the carrageenan-induced pleurisy model, the RDV-8 (3 mg/kg) treatment induced a significant reduction in the exudate volume, in the polymorphonuclear leukocyte migration and in the pleural exudate NO levels. The results indicate that RDV-8 may have an immunomodulatory effect, as well as anti-inflammatory actions suggesting that it could represent a new strategy in the inflammatory response modulation.

• Authors: Marcos Schuch Azambuja, Adroaldo Lunardelli, Robson Henrich Amaral, Fernanda Bordignon Nunes, Eduardo Caberlon, Vinicius Lorini da Costa, Márcio Vinícius Fagundes Donadio, Daniela Nunes Vitor, Alberto da Silva Melo Denizar, Silvio Cunha, Jarbas Rodrigues de Oliveira
• Journal: Inflammopharmacology (Inflammopharmacology) Vol. 19 Issue 3 Pg. 145-53 (Jun 2011) ISSN: 1568-5608 [Electronic] Switzerland
• PMID: 20981574 (Publication Type: Journal Article)

Chemical References

• Anti-Inflammatory Agents
• Chemokine CCL2
• Immunologic Factors
• Pyrimidines
• Thiones
• ethyl 1-butyl-6-methyl-2-phenyl-4-thioxo-1,4-dihydropyrimidine-5-carboxylate
• Nitric Oxide
• Carrageenan

Topics

• Animals
• Anti-Inflammatory Agents (administration & dosage, pharmacology)
• Carrageenan
• Cell Movement (drug effects)
• Cell Proliferation (drug effects)
• Chemokine CCL2 (biosynthesis, drug effects)
• Disease Models, Animal
• Dose-Response Relationship, Drug
• Female
• Humans
• Immunologic Factors (administration & dosage, pharmacology)
• Leukocytes, Mononuclear (drug effects, metabolism)
• Neutrophils (drug effects, metabolism)
• Nitric Oxide (metabolism)
• Pleurisy (drug therapy, physiopathology)
• Pyrimidines (administration & dosage, pharmacology)
• Rats
• Rats, Wistar
• Thiones (administration & dosage, pharmacology)