Abstract | BACKGROUND: METHODS: To evaluate the effect of long-term alcohol intake, rats were fed either a Lieber-DeCarli control diet (CD) or a Lieber-DeCarli alcohol diet (AD) for 6 weeks. Then, rats were infused over 2 h with either Ringer's solution (CO) or ethanol (E). In additional animals, alcoholic pancreatitis was induced by ethanol combined with hyperlipidemia and temporary pancreatic duct obstruction (EFO). Controls received Ringer's solution combined with hyperlipidemia and temporary pancreatic duct obstruction (RFO). Intravital microscopy (pancreatic perfusion and leukocyte adhesion), alcohol concentrations, amylase, lipase, cholesterine and triglyceride levels in plasma, myeloperoxidase activity and histology were evaluated at different time intervals. RESULTS: In those animals which received the Lieber-DeCarli control diet, capillary perfusion was reduced in the E group and further reduced in the EFO group as compared to the controls (CO, RFO; p < 0.01). Leukocyte adhesion was significantly increased in rats receiving E (p < 0.01), and was further increased in the combination group EFO (p < 0.01). EFO induced histologically evident acute pancreatitis. The additional administration of a long-term alcohol diet further increased microcirculatory disturbances and pancreatic injury significantly (EFO-AD > EFO-CD). CONCLUSIONS:
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Authors | J Grauvogel, T D Daemmrich, E Ryschich, M M Gebhard, J Werner |
Journal | Pancreatology : official journal of the International Association of Pancreatology (IAP) ... [et al.]
(Pancreatology)
Vol. 10
Issue 5
Pg. 603-12
( 2010)
ISSN: 1424-3911 [Electronic] Switzerland |
PMID | 20980778
(Publication Type: Journal Article)
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Copyright | Copyright © 2010 S. Karger AG, Basel. |
Topics |
- Alcohol Drinking
- Alcoholism
(complications, pathology)
- Animals
- Hyperlipidemias
(complications, pathology)
- Ligation
- Male
- Microcirculation
(drug effects)
- Pancreas
(blood supply)
- Pancreatic Ducts
(pathology, surgery)
- Pancreatitis, Alcoholic
(chemically induced, pathology)
- Rats
- Rats, Wistar
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