Detection of Candida by calcofluor white.

Abstract

OBJECTIVE:

To assess the efficacy of calcofluor white (CFW) is compared to Gram stain and periodic acid-Schiff in detection of Candida in oral precancer and cancer.

STUDY DESIGN:

The study group consisted of patients with precancer (n = 45), cancer (n = 45) and control group (n = 45). Presence of Candida was confirmed by culture inoculation along with a germ tube and carbohydrate fermentation test. The cytopathologic smears were analyzed by Papanicolaou-CFW and Gram staining, whereas, tissue sections were stained by periodic acid-Schiff and CFW staining.

RESULTS:

Candida albicans was the predominant species identified. A highly significant association of Candida was seen more often in cancer than in precancer. Both in cytology and histopathology Candida detection by CFW was higher. In precancer it was 48.88% in smears and 40% in tissue sections, whereas, in cancer 60% in smears and 55.55% in histopathology.

CONCLUSION:

Among the various diagnostic tools used in the present study, the use of CFW is seen to be a simple, effective, rapid and reliable method, both in cytopathology and histopathology.

• Authors: Rashmi S K Bhavasar, Santosh Kumar Goje, Anant Arunrao Takalkar, Sindhu M Ganvir, Vinay K Hazarey, Suchitra R Gosavi
• Journal: Acta cytologica (Acta Cytol) 2010 Sep-Oct Vol. 54 Issue 5 Pg. 679-84 ISSN: 0001-5547 [Print] Switzerland
• PMID: 20968155 (Publication Type: Journal Article)

Chemical References

• Benzenesulfonates
• Fluorescent Dyes
• Gram's stain
• Phenazines
• C.I. Fluorescent Brightening Agent 28
• Gentian Violet

Topics

• Adolescent
• Adult
• Benzenesulfonates (metabolism)
• Candida (metabolism)
• Candidiasis, Oral (metabolism, microbiology, pathology)
• Child
• Child, Preschool
• Female
• Fluorescent Dyes (metabolism)
• Gentian Violet
• Humans
• Infant
• Infant, Newborn
• Leukoplakia, Oral (microbiology, pathology)
• Lichen Planus, Oral (microbiology, pathology)
• Male
• Microscopy, Fluorescence
• Middle Aged
• Mouth Neoplasms (metabolism, microbiology, pathology)
• Oral Submucous Fibrosis (microbiology, pathology)
• Phenazines
• Sensitivity and Specificity
• Staining and Labeling
• Young Adult