Stamp wound assay for studying coupled cell migration and cell debris clearance.

Abstract

A new method for studying wound healing under realistic conditions in vitro was developed. The method involves creating defined patterns of damaged cell debris with poly(dimethyl)siloxane (PDMS) stamping. This novel assay permitted the quantification of wound healing rates in the presence of cell debris. Experimental results with this assay suggest that cell migration in the presence of cell debris is a two step process requiring (1) non-muscle myosin II-dependent cell clearance followed by (2) cell migration into newly cleared wound areas. The novel stamp wound assay allows the study of coupled cell migration and debris clearance and is a more realistic wound healing assay in vitro.

• Authors: Jiyeon Lee, Yu-Lin Wang, Fan Ren, Tanmay P Lele
• Journal: Langmuir : the ACS journal of surfaces and colloids (Langmuir) Vol. 26 Issue 22 Pg. 16672-6 (Nov 16 2010) ISSN: 1520-5827 [Electronic] United States
• PMID: 20961056 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S.)

Chemical References

• Dimethylpolysiloxanes
• Heterocyclic Compounds, 4 or More Rings
• blebbistatin
• baysilon
• Myosin Type II

Topics

• Cell Death (drug effects)
• Cell Line
• Cell Movement (drug effects)
• Cells, Immobilized (cytology, drug effects)
• Dimethylpolysiloxanes (chemistry)
• Epithelial Cells (cytology, drug effects)
• Heterocyclic Compounds, 4 or More Rings (pharmacology)
• Humans
• Myosin Type II (antagonists & inhibitors)
• Time Factors
• Wound Healing (drug effects)