Abstract | OBJECTIVES/HYPOTHESIS: STUDY DESIGN: Prospective experimental study, cross-sectional analysis. METHODS: Relative changes in amphiregulin gene expression were quantitated by real-time reverse-transcription polymerase chain reaction analyses of cholesteatoma epithelium compared to uninvolved control tissues from patients' postauricular and external auditory canal regions. Western immunoblot assays were performed for qualitative evaluation of amphiregulin protein expression. The t test and Fisher exact test were used for analysis. RESULTS: A statistically significant increase in amphiregulin gene expression was associated with cholesteatoma specimens compared to uninvolved postauricular skin (PAS) and external auditory canal (EAC) skin, P = .004 and P = .002, respectively. From comparisons of 60 sets of skin pairs, the mean ratio of amphiregulin RNA expression for cholesteatoma/PAS is 4.94 (standard error of the mean [SEM] = 1.53, n = 30) and for cholesteatoma/EAC is 7.70 (SEM = 1.57, n = 30). CONCLUSIONS:
|
Authors | MiMi P Macias, Richard D Gerkin, John D Macias |
Journal | The Laryngoscope
(Laryngoscope)
Vol. 120
Issue 11
Pg. 2258-63
(Nov 2010)
ISSN: 1531-4995 [Electronic] United States |
PMID | 20938960
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
|
Chemical References |
- AREG protein, human
- Amphiregulin
- Biomarkers
- EGF Family of Proteins
- Glycoproteins
- Intercellular Signaling Peptides and Proteins
|
Topics |
- Adolescent
- Adult
- Aged
- Amphiregulin
- Biomarkers
(metabolism)
- Blotting, Western
- Child
- Cholesteatoma
(genetics, pathology, surgery)
- Cholesteatoma, Middle Ear
(genetics, pathology)
- Cohort Studies
- EGF Family of Proteins
- Female
- Gene Expression Regulation
- Glycoproteins
(metabolism)
- Humans
- Intercellular Signaling Peptides and Proteins
(metabolism)
- Male
- Middle Aged
- Prospective Studies
- Reference Values
- Reverse Transcriptase Polymerase Chain Reaction
- Statistics, Nonparametric
- Temporal Bone
- Young Adult
|