Abstract | BACKGROUND: METHODS: Human HGF gene was transferred into MSCs in vitro. The HGF gene-modified MSCs were transplanted by means of intrathecal injection. Two days later, spinal cord ischemia was induced by occlusion of the infrarenal aorta with a balloon catheter for 40 or 50 min. Hind-limb motor function was assessed during a 14-day recovery period with Tarlov criteria, and then histologic examination was performed. RESULTS: Human HGF was detected in the cerebrospinal fluid from 2 to 16 days after transplantation of HGF gene-modified MSCs. Compared with the controls, transplantation of HGF gene-modified MSCs or MSCs alone significantly improved the Tarlov scores 1, 2, 7, and 14 days after spinal cord ischemia of 40 or 50 min (P < 0.01, respectively) and increased the number of intact motor neurons in the lumbar spinal cord (P < 0.01, respectively). When the ischemic period was extended to 50 min, the Tarlov scores and the number of intact motor neurons of rabbits transplanted with HGF gene-modified MSCs were markedly higher than those of the rabbits transplanted with MSCs only (P < 0.05, respectively). CONCLUSIONS:
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Authors | Enyi Shi, Xiaojing Jiang, Lingling Wang, Satoshi Akuzawa, Yoshiki Nakajima, Teruhisa Kazui |
Journal | Anesthesiology
(Anesthesiology)
Vol. 113
Issue 5
Pg. 1109-17
(Nov 2010)
ISSN: 1528-1175 [Electronic] United States |
PMID | 20930612
(Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- HGF protein, human
- Neuroprotective Agents
- Hepatocyte Growth Factor
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Topics |
- Animals
- Bone Marrow Cells
- Bone Marrow Transplantation
(methods)
- Cells, Cultured
- Hepatocyte Growth Factor
(administration & dosage, genetics)
- Humans
- Injections, Spinal
- Neuroprotective Agents
(administration & dosage)
- Rabbits
- Random Allocation
- Reperfusion Injury
(pathology, prevention & control, surgery)
- Spinal Cord Ischemia
(pathology, prevention & control, surgery)
- Stromal Cells
(transplantation)
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