A two-component histidine kinase, MoSLN1, is required for cell wall integrity and pathogenicity of the rice blast fungus, Magnaporthe oryzae.

A two-component signal transduction system is a common mechanism for environmental sensing in bacteria. The functions of the two-component molecules have been also well characterized in the lower eukaryotic fungi in recent years. In Saccharomyces cerevisiae, the histidine kinase Sln1p is a major component of the two-component signaling pathways and a key regulator of the osmolarity response. To determine the function of MoSLN1, a Sln1 homolog of Magnaporthe oryzae, we cloned the MoSLN1 gene and generated specific mutants using gene knock-out strategy. Disruption of MoSLN1 resulted in hypersensitivity to various stresses, reduced sensitivity to cell wall perturbing agent Calcofluor white, and loss of pathogenicity, mainly due to a penetration defect. Additionally, we showed that MoSLN1 is involved in oxidative signaling through modulation of intra- and extracellular peroxidase activities. These results indicate that MoSLN1 functions as a pathogenicity factor that plays a role in responses to osmotic stress, the cell wall integrity, and the activity of peroxidases.
AuthorsHaifeng Zhang, Kaiyue Liu, Xing Zhang, Wenwen Song, Qian Zhao, Yanhan Dong, Min Guo, Xiaobo Zheng, Zhengguang Zhang
JournalCurrent genetics (Curr Genet) Vol. 56 Issue 6 Pg. 517-28 (Dec 2010) ISSN: 1432-0983 [Electronic] United States
PMID20848286 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Fungal Proteins
  • Superoxides
  • Protein Kinases
  • protein-histidine kinase
  • Cell Membrane Permeability (genetics)
  • Cell Wall (metabolism, physiology)
  • Cloning, Molecular
  • Fungal Proteins (genetics)
  • Magnaporthe (enzymology, genetics, metabolism, pathogenicity)
  • Models, Biological
  • Organisms, Genetically Modified
  • Oryza (microbiology)
  • Osmotic Pressure (physiology)
  • Phylogeny
  • Plant Diseases (genetics, microbiology)
  • Protein Kinases (genetics, metabolism, physiology)
  • Superoxides (metabolism)

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