[Effects of Ginkgolide B on inflammation induced by cerebral ischemia-reperfusion in rats].

Abstract

OBJECTIVE:

To investigate the protective effects of Ginkgolide B on inflammation induced by cerebral ischemia-reperfusion in rats.

METHODS:

Rats were pretreated with Ginkgolide B at the dose of 2. 5, 5, 10 mg/kg for 3 days and then subjected to cerebral ischemia/reperfusion induced by a middle cerebral artery occlusion (MCAO). The infarct volume and the neurological deficit were determined by the method of TTC (2,3,5-triphenylterazolium chloride) staining and Longa's score. The permeability of blood-brain barrier (BBB) was evaluated by measurement of the evans blue (EB) content in the brain with spectrophotometer. The content of interleukin-1beta, interleukin-6 (IL-6, IL-1beta) in serum and tumor necrosis factor-alpha (TNF-alpha), The content of E-selectin and ICAM 1 in brain were determined by radio-immunoassay and ELISA assay.

RESULTS:

Ginkgolide B reduced infarct volume, ameliorated the neurological deficit and the permeability of BBB, the content of IL-6, IL-1beta in serum and TNF-alpha, expressions of E-selectin and ICAM 1 in brain tissue also were significantly decreased.

CONCLUSION:

Ginkgolide B has protective effects on cerebral injury by inhibiting the inflammation induced by ischemia/reperfusion injury.

• Authors: Yong-Gang Liu, Fang-Jun Li, Jing Wang, Xiao-Dong Wang
• Journal: Zhong yao cai = Zhongyaocai = Journal of Chinese medicinal materials (Zhong Yao Cai) Vol. 33 Issue 4 Pg. 578-80 (Apr 2010) ISSN: 1001-4454 [Print] China
• PMID: 20845788 (Publication Type: Journal Article)

Chemical References

• E-Selectin
• Ginkgolides
• Inflammation Mediators
• Interleukin-1beta
• Interleukin-6
• Lactones
• Neuroprotective Agents
• Tumor Necrosis Factor-alpha
• ginkgolide B

Topics

• Animals
• Blood-Brain Barrier (drug effects, pathology)
• Brain (drug effects, metabolism, pathology)
• Brain Ischemia (complications)
• Disease Models, Animal
• E-Selectin (metabolism)
• Ginkgo biloba (chemistry)
• Ginkgolides (administration & dosage, pharmacology)
• Infarction, Middle Cerebral Artery (blood, etiology, prevention & control)
• Inflammation Mediators (blood, metabolism)
• Interleukin-1beta (blood)
• Interleukin-6 (blood)
• Lactones (administration & dosage, pharmacology)
• Male
• Neuroprotective Agents (pharmacology)
• Plant Leaves (chemistry)
• Random Allocation
• Rats
• Rats, Sprague-Dawley
• Reperfusion Injury (complications)
• Tumor Necrosis Factor-alpha (metabolism)