Immune sera from 15 patients with cured
amoebic liver abscess were used to recognise the
antigens of Entamoeba histolytica (HMI) by immunoblotting. The amoebic
proteins most frequently recognised by sera from patients with cured
amoebic liver abscess had molecular masses of 8, 13, 18, 22, 29, 38, 45, 67 and 94 kDa. Six plasma membrane-associated amoebic
proteins of molecular mass 29, 38, 45-67 complex, 85 and 94 kDa were strongly recognised by such sera. Two plasma membrane-associated
antigens of 108 and 129 kDa were not recognised by any sera. None of the crude or plasma membrane-associated
antigens were recognised by sera from five patients of idiopathic
ulcerative colitis, five patients of persistent
giardiasis and five normal healthy subjects. Identification of such
antigens, especially plasma membrane-associated
antigens may pave a way to develop specific diagnostic and immunoprotective agents.