Abstract |
DNA vaccines contribute to a promising new approach for the generation of cytotoxic T lymphocytes (CTL). DNA vaccines do have several disadvantages, including poor immunogenicity and oncogene expression. We used the natural killer T-cell (NKT) ligand α- galactosylceramide (α-GalCer) as an adjuvant to prime initial DNA vaccination; and used the potent immune-stimulatory tumor antigen-expressing dendritic cells (DCs) as a booster vaccination. A DNA vaccine expressing human papillomavirus (HPV) type 16 E7 (pcDNA3-CRT/E7) was combined with α-GalCer at the prime phase, and generated a higher number of E7-specific CD8(+) T-cells in vaccinated mice than vaccine used at boost phase. Therefore, priming with a DNA vaccine in the presence of α-GalCer and boosting with E7-pulsed DC-1 led to a significant enhancement of E7-specific CD8(+) effector and memory T-cells as well as significantly improved therapeutic and preventive effects against an E7-expressing tumor model (TC-1) in vaccinated mice. Our findings suggested that the potency of a DNA vaccine combined with α-GalCer could be further enhanced by boosting with an antigen-expressing DC-based vaccine to generate anti- tumor immunity.
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Authors | Daejin Kim, Chien-Fu Hung, T-C Wu, Yeong-Min Park |
Journal | Vaccine
(Vaccine)
Vol. 28
Issue 45
Pg. 7297-305
(Oct 21 2010)
ISSN: 1873-2518 [Electronic] Netherlands |
PMID | 20817010
(Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2010 Elsevier Ltd. All rights reserved. |
Chemical References |
- Adjuvants, Immunologic
- Cancer Vaccines
- Cytokines
- Galactosylceramides
- Papillomavirus E7 Proteins
- Vaccines, DNA
- alpha-galactosylceramide
- oncogene protein E7, Human papillomavirus type 16
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Topics |
- Adjuvants, Immunologic
(administration & dosage)
- Animals
- CD8-Positive T-Lymphocytes
(immunology)
- Cancer Vaccines
(immunology)
- Cell Line, Tumor
- Cytokines
(immunology)
- Dendritic Cells
(immunology)
- Female
- Galactosylceramides
(administration & dosage, immunology)
- Immunization, Secondary
- Mice
- Mice, Inbred C57BL
- Neoplasms, Experimental
(immunology, prevention & control)
- Papillomavirus E7 Proteins
(immunology)
- Spleen
(cytology)
- Vaccines, DNA
(immunology)
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