Abstract | OBJECTIVE: The ischemic myocardium releases multiple chemotactic factors responsible for the mobilization and recruitment of bone marrow-derived cells to injured myocardium. However, the mobilization of primitive pluripotent stem cells (PSCs) enriched in very small embryonic-like stem cells (VSELs) in various cardiac ischemic scenarios is not well understood. MATERIALS AND METHODS: RESULTS: The absolute numbers of PSCs, stem cell populations enriched in VSELs, and hematopoietic stem cells present in PB were significantly higher in STEMI patients at presentation and declined over time. There was a corresponding increase in pluripotent, cardiac, and endothelial gene expression in unfractionated PB cells and sorted PB-derived primitive CD34(+) cells. The absolute numbers of circulating VSELs and hematopoietic stem cells in STEMI correlated negatively with patient age. CONCLUSIONS:
Myocardial ischemia mobilizes primitive PSCs including pluripotent VSELs into the circulation. The peak of mobilization occurs within 12 hours in patients presenting with STEMI, which may represent a therapeutic window for future clinical applications. Reduced stem cell mobilization with advancing age could explain, in part, the observation that age is associated with poor prognosis in patients with myocardial infarction.
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Authors | Ahmed Abdel-Latif, Ewa K Zuba-Surma, Khaled M Ziada, Magdalena Kucia, Donald A Cohen, Alan M Kaplan, Gary Van Zant, Samy Selim, Susan S Smyth, Mariusz Z Ratajczak |
Journal | Experimental hematology
(Exp Hematol)
Vol. 38
Issue 12
Pg. 1131-1142.e1
(Dec 2010)
ISSN: 1873-2399 [Electronic] Netherlands |
PMID | 20800644
(Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)
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Copyright | Published by Elsevier Inc. |
Chemical References |
- Cytokines
- Octamer Transcription Factor-3
- POU5F1 protein, human
- Stage-Specific Embryonic Antigens
- stage-specific embryonic antigen-4
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Topics |
- Adult
- Age Factors
- Aged
- Cytokines
(blood)
- Female
- Flow Cytometry
- Hematopoietic Stem Cell Mobilization
- Humans
- Immunophenotyping
- Male
- Middle Aged
- Myocardial Ischemia
(blood, therapy)
- Octamer Transcription Factor-3
(analysis)
- Pluripotent Stem Cells
(physiology)
- Reverse Transcriptase Polymerase Chain Reaction
- Stage-Specific Embryonic Antigens
(analysis)
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