[Effect of photodynamic therapy on the proliferation and cell distribution of hypertrophic scar fibroblast].

Abstract

OBJECTIVE:

To investigate the effect of HMME-PDT on the proliferation and cell distribution of hypertrophic scar fibroblast (HSF).

METHODS:

HSF were cultured and only 4-6th passages were used in this study. Argyrophilic protein in nucleolar organizer regions(AgNORs) were calculated by I. S% after argyrophilic staining. Flow cytometry was applied to analyze the cell cycle and proliferation index (PI).

RESULTS:

1) I. S% of HSF after HMME-PDT was reduced markedly. 2) HMME-PDT inhibited HSF entering S stage from G, stage, cell percentage in S stage was decreased to (11.2 +/- 2.3)%. 3) PI in HMME-PDT group was less than that in control group [(35.0 +/- 3.4)% vs (27.2 +/- 3.1)%, P < 0.05].

CONCLUSIONS:

HMME-PDT can inhibit proliferation of HSF, and chang cell distribution.

• Authors: Hong Cai, Ying Gu, Qiang Sun, Jing Zeng, Shi-Long Li
• Journal: Zhonghua zheng xing wai ke za zhi = Zhonghua zhengxing waike zazhi = Chinese journal of plastic surgery (Zhonghua Zheng Xing Wai Ke Za Zhi) Vol. 26 Issue 3 Pg. 212-5 (May 2010) ISSN: 1009-4598 [Print] China
• PMID: 20737952 (Publication Type: English Abstract, Journal Article)

Topics

• Cell Cycle (drug effects)
• Cell Proliferation (drug effects)
• Cells, Cultured
• Cicatrix (drug therapy, pathology)
• Fibroblasts (drug effects, metabolism, pathology)
• Humans
• Photochemotherapy