Abstract |
The purpose of the present study was to evaluate the clinical usefulness of detection of serum immunoglobulin A ( IgA), IgG, and IgM antibodies raised against the mycobacterial A60 antigen for the diagnosis and discrimination of active tuberculosis (TB) from other pulmonary diseases. Three commercially available ELISA kits ( IgA, IgG, and IgM) (ANDA Biologicals, Strasbourg, France) were evaluated simultaneously in 246 serum samples from 3 groups of patients: group I, 171 patients with active TB (128 pulmonary TB and 43 extrapulmonary TB); group II, 73 patients with pulmonary non-TB diseases; and group III, 2 leprosies patients. The sensitivities of tests ranged from 31.3% ( IgA) to 94% ( IgG) in pulmonary TB patients and from 21% ( IgA) to 84% ( IgG) in extrapulmonary TB patients. The specificities of assays varied from 92% ( IgG) to 96% ( IgA) in the pulmonary non-TB group. Combination of IgG with IgA and/or IgM does not improve its sensitivity. Clinical use of the A60-based serodiagnostic IgG assay is of great value for the rapid diagnosis and discrimination between active TB and pulmonary non-TB diseases. Moreover, this test could be used to increase diagnostic accuracy, especially for smear-negative TB cases, which are difficult to diagnose.
|
Authors | Walid Ben-selma, Hedi Harizi, Manel Marzouk, Imen Ben Kahla, Foued Ben Lazreg, Asma Ferjeni, Jalel Boukadida |
Journal | Diagnostic microbiology and infectious disease
(Diagn Microbiol Infect Dis)
Vol. 68
Issue 1
Pg. 55-9
(Sep 2010)
ISSN: 1879-0070 [Electronic] United States |
PMID | 20727471
(Publication Type: Evaluation Study, Journal Article, Research Support, Non-U.S. Gov't)
|
Chemical References |
- Antibodies, Bacterial
- Antigens, Bacterial
- Immunoglobulin A
- Immunoglobulin G
- Immunoglobulin M
- mycobacterial A60 antigen
|
Topics |
- Antibodies, Bacterial
(blood)
- Antigens, Bacterial
(immunology)
- Enzyme-Linked Immunosorbent Assay
- Humans
- Immunoglobulin A
(blood)
- Immunoglobulin G
(blood)
- Immunoglobulin M
(blood)
- Mycobacterium tuberculosis
(immunology)
- Predictive Value of Tests
- Sensitivity and Specificity
- Tuberculosis
(diagnosis, immunology, microbiology)
- Tuberculosis, Pulmonary
(diagnosis, immunology, microbiology)
|