Abstract | OBJECTIVES: MATERIALS AND METHODS: To analyze effects of hypothermia on the Galalpha1-3Galbeta1-4GlcNAc-Rs, cultured porcine aortic endothelial cells were exposed to a temperature of 4 degrees C for 1 hour, 4 hours, and 6 hours. Cell cultures of the control groups were incubated at the same time at 38 degrees C. To investigate the influence of cardioplegic solutions on the Galalpha1- 3Galbeta1-4GlcNAc-Rs, porcine aortic endothelial cells were exposed to 4 degrees C for 4 hours in the presence of University of Wisconsin solution or histidinetryptophan- ketoglutarate solution. Cells of the control groups were cooled at 4 degrees C for 4 hours without cardioplegic solution. After treatment, porcine aortic endothelial cells were submitted to fluorescence-activated cell sorter. RESULTS:
Hypothermia of 4 degrees C showed no significant effect on the quantity of Galalpha1-3Galbeta1-4GlcNAc-Rs. However, the treatment of porcine aortic endothelial cells with University of Wisconsin solution resulted in a highly significant reduction of Galalpha1-3Galbeta1- 4GlcNAc-Rs by 50% (P = .006). Treatment of porcine aortic endothelial cells with histidine-tryptophanketoglutarate solution decreased Alpha-Gal quantity significantly by 32% (P = .011). CONCLUSIONS: Our data offer new perspectives in the prevention of hyperacute, humoral xenograft rejection by reducing the Galalpha1-3Galbeta1-4GlcNAc-Rs after exposure to different cardioplegic solutions.
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Authors | Miriam Keller, Andres Beiras-Fernandez, Michael Schmoeckel, Bruno Reichart, Paolo Brenner |
Journal | Experimental and clinical transplantation : official journal of the Middle East Society for Organ Transplantation
(Exp Clin Transplant)
Vol. 8
Issue 3
Pg. 250-7
(Sep 2010)
ISSN: 2146-8427 [Electronic] Turkey |
PMID | 20716045
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antigens, Heterophile
- Bretschneider cardioplegic solution
- Cardioplegic Solutions
- Insulin
- Organ Preservation Solutions
- Trisaccharides
- University of Wisconsin-lactobionate solution
- alpha-galactosyl epitope
- Mannitol
- Procaine
- Allopurinol
- Potassium Chloride
- Glutathione
- Glucose
- Adenosine
- Raffinose
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Topics |
- Adenosine
(pharmacology)
- Allopurinol
(pharmacology)
- Animals
- Antigens, Heterophile
(metabolism)
- Aorta
(drug effects, immunology)
- Cardioplegic Solutions
(pharmacology)
- Cell Separation
(methods)
- Cells, Cultured
- Down-Regulation
- Endothelial Cells
(drug effects, immunology)
- Flow Cytometry
- Glucose
(pharmacology)
- Glutathione
(pharmacology)
- Graft Rejection
(immunology, prevention & control)
- Hypothermia, Induced
- Immunity, Humoral
(drug effects)
- Insulin
(pharmacology)
- Mannitol
(pharmacology)
- Organ Preservation Solutions
(pharmacology)
- Potassium Chloride
(pharmacology)
- Procaine
(pharmacology)
- Raffinose
(pharmacology)
- Swine
- Time Factors
- Trisaccharides
(metabolism)
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