Abstract | BACKGROUND: Cardiac cell transplantation is compromised by low cell retention and poor graft viability. Here, the effects of co-injecting adipose tissue-derived stem cells (ASCs) with biopolymers on cell cardiac retention, ventricular morphometry and performance were evaluated in a rat model of myocardial infarction (MI). METHODOLOGY/PRINCIPAL FINDINGS: 99mTc-labeled ASCs (1x10(6) cells) isolated from isogenic Lewis rats were injected 24 hours post-MI using fibrin a, collagen (ASC/C), or culture medium (ASC/M) as vehicle, and cell body distribution was assessed 24 hours later by gamma-emission counting of harvested organs. ASC/F and ASC/C groups retained significantly more cells in the myocardium than ASC/M (13.8+/-2.0 and 26.8+/-2.4% vs. 4.8+/-0.7%, respectively). Then, morphometric and direct cardiac functional parameters were evaluated 4 weeks post-MI cell injection. Left ventricle (LV) perimeter and percentage of interstitial collagen in the spare myocardium were significantly attenuated in all ASC-treated groups compared to the non-treated (NT) and control groups (culture medium, fibrin, or collagen alone). Direct hemodynamic assessment under pharmacological stress showed that stroke volume (SV) and left ventricle end-diastolic pressure were preserved in ASC-treated groups regardless of the vehicle used to deliver ASCs. Stroke work (SW), a global index of cardiac function, improved in ASC/M while it normalized when biopolymers were co-injected with ASCs. A positive correlation was observed between cardiac ASCs retention and preservation of SV and improvement in SW post-MI under hemodynamic stress. CONCLUSIONS: We provided direct evidence that intramyocardial injection of ASCs mitigates the negative cardiac remodeling and preserves ventricular function post-MI in rats and these beneficial effects can be further enhanced by administering co-injection of ASCs with biopolymers.
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Authors | Maria E Danoviz, Juliana S Nakamuta, Fabio L N Marques, Leonardo dos Santos, Erica C Alvarenga, Alexandra A dos Santos, Ednei L Antonio, Isolmar T Schettert, Paulo J Tucci, Jose E Krieger |
Journal | PloS one
(PLoS One)
Vol. 5
Issue 8
Pg. e12077
(Aug 10 2010)
ISSN: 1932-6203 [Electronic] United States |
PMID | 20711471
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Biopolymers
- Fibrin
- Collagen
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Topics |
- Adipose Tissue
(cytology)
- Animals
- Biopolymers
(administration & dosage, pharmacology)
- Cell Survival
(drug effects)
- Cells, Cultured
- Collagen
(administration & dosage, pharmacology)
- Female
- Fibrin
(administration & dosage, pharmacology)
- Heart
(drug effects, physiopathology)
- Injections
- Myocardial Infarction
(metabolism, pathology, physiopathology, surgery)
- Myocardium
(metabolism, pathology)
- Rats
- Rats, Inbred Lew
- Stem Cell Transplantation
- Stem Cells
(cytology, drug effects, metabolism)
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