Abstract |
We describe a new cable-free, non-telemetric method for synchronized electrophysiological and video recordings of seizure activity in freely moving mice. The electrophysiological recordings were made by a head-mounted 4-channel data-logging device, allowing the mouse to move freely in its cage, and even to be moved from cage to cage under ongoing recording. Seizures were studied in Synapsin I/II double knock-out (SynDKO) mice, a genetically engineered mouse line that shows seizures upon daily handling procedures such as tail lifting during cage changes, much in resemblance to the more studied El mouse. The ability to elicit seizures through daily handling in SynDKO mice undergoing electrophysiological recording is a significant improvement in comparison to the traditional cable-based set-up. Furthermore, with its four channels and a sample rate of up to 500Hz, the data-logging device opens for more varied electrophysiological studies than other available cable-free systems.
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Authors | Lars Etholm, Dimitrula Arabadzisz, Hans-Peter Lipp, Paul Heggelund |
Journal | Journal of neuroscience methods
(J Neurosci Methods)
Vol. 192
Issue 2
Pg. 254-60
(Oct 15 2010)
ISSN: 1872-678X [Electronic] Netherlands |
PMID | 20708034
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2010 Elsevier B.V. All rights reserved. |
Topics |
- Animals
- Data Collection
- Electroencephalography
(methods)
- Electronic Data Processing
- Mice
- Mice, Knockout
- Seizures
(physiopathology)
- Video Recording
(methods)
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