Entrapment neuropathy results in different microRNA expression patterns from denervation injury in rats.
Abstract | BACKGROUND: METHODS: The experimental L4-L6 spinal segments, dorsal root ganglia (DRGs), and soleus muscles from each experimental group ( sham control, denervation, entrapment, and decompression) were analyzed using an Agilent rat miRNA array to detect dysregulated miRNAs. In addition, muscle-specific miRNAs (miR-1, -133a, and -206) and selectively upregulated miRNAs were subsequently quantified using real-time reverse transcription-polymerase chain reaction (real-time RT-PCR). RESULTS: In the soleus muscles, 37 of the 47 miRNAs (13.4% of the 350 unique miRNAs tested) that were significantly downregulated after 6 months of entrapment neuropathy were also among the 40 miRNAs (11.4% of the 350 unique miRNAs tested) that were downregulated after 3 months of decompression. No miRNA was upregulated in both groups. In contrast, only 3 miRNAs were upregulated and 3 miRNAs were downregulated in the denervated muscle after 6 months. In the DRGs, 6 miRNAs in the entrapment group (miR-9, miR-320, miR-324-3p, miR-672, miR-466b, and miR-144) and 3 miRNAs in the decompression group (miR-9, miR-320, and miR-324-3p) were significantly downregulated. No miRNA was upregulated in both groups. We detected 1 downregulated miRNA (miR-144) and 1 upregulated miRNA (miR-21) after sciatic nerve denervation. We were able to separate the muscle or DRG samples into denervation or entrapment neuropathy by performing unsupervised hierarchal clustering analysis. Regarding the muscle-specific miRNAs, real-time RT-PCR analysis revealed an approximately 50% decrease in miR-1 and miR-133a expression levels at 3 and 6 months after entrapment, whereas miR-1 and miR-133a levels were unchanged and were decreased after decompression at 1 and 3 months. In contrast, there were no statistical differences in the expression of miR-206 during nerve entrapment and after decompression. The expression of muscle-specific miRNAs in entrapment neuropathy is different from our previous observations in sciatic nerve denervation injury. CONCLUSIONS: This study revealed the different involvement of miRNAs in neurons and innervated muscles after entrapment neuropathy and denervation injury, and implied that epigenetic regulation is different in these two conditions.
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Authors | Cheng-Shyuan Rau, Jonathan Chris Jeng, Seng-Feng Jeng, Tsu-Hsiang Lu, Yi-Chun Chen, Po-Chou Liliang, Chia-Jung Wu, Chia-Jung Lin, Ching-Hua Hsieh |
Journal | BMC musculoskeletal disorders
(BMC Musculoskelet Disord)
Vol. 11
Pg. 181
(Aug 12 2010)
ISSN: 1471-2474 [Electronic] England |
PMID | 20704709
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- MIRN133 microRNA, rat
- MicroRNAs
- mirn206 microRNA, rat
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Topics |
- Animals
- Denervation
- Disease Models, Animal
- Down-Regulation
(genetics)
- Ganglia, Spinal
(metabolism, physiopathology)
- Gene Expression Regulation
(genetics)
- Male
- MicroRNAs
(antagonists & inhibitors, biosynthesis)
- Muscle, Skeletal
(innervation, metabolism, physiopathology)
- Nerve Compression Syndromes
(genetics, metabolism, physiopathology)
- Neurons
(metabolism, pathology)
- Peripheral Nervous System Diseases
(genetics, metabolism, physiopathology)
- Rats
- Rats, Sprague-Dawley
- Sciatic Neuropathy
(genetics, metabolism, physiopathology)
- Spinal Cord
(metabolism, physiopathology)
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