Therapy-related, mixed phenotype acute leukemia with t(1;21)(p36;q22) and RUNX1 rearrangement.

Abstract

We describe here a new case of therapy-related acute leukemia with t(1;21)(p36;q22). A 25-year-old man was admitted because of anemia and thrombocytopenia. Four years before, he had received combination chemotherapy including etoposide for seminoma. Bone marrow was hypercellular, with 49% myeloperoxidase (MPO) staining-negative blasts. Chromosome analysis showed 46,XY,t(1;21)(p36.3;q22)[11]/49,sl,+8,+16,+20[9]. Fluorescence in situ hybridization demonstrated that RUNX1 signals at 21q22 were split onto the der(1)t(1;21) and der(21)t(1;21). Immunophenotypic analyses revealed that blasts were positive for CD19, CD79a, and cytCD22, as well as MPO, CD13, and CD33, fulfilling the diagnostic criteria of mixed phenotype acute leukemia, B/myeloid. The patient died of disease progression after 10 months. Thus, acute leukemia with t(1;21) and RUNX1 rearrangement could be associated with B/myeloid mixed phenotype as well as previous topoisomerase II inhibitor therapy and poor prognoses.

• Authors: Katsuya Yamamoto, Akiko Sada, Yuko Kawano, Yoshio Katayama, Manabu Shimoyama, Toshimitsu Matsui
• Journal: Cancer genetics and cytogenetics (Cancer Genet Cytogenet) Vol. 201 Issue 2 Pg. 122-7 (Sep 2010) ISSN: 1873-4456 [Electronic] United States
• PMID: 20682397 (Publication Type: Case Reports, Journal Article)
• Copyright: 2010 Elsevier Inc. All rights reserved.

Chemical References

• Antineoplastic Agents
• Core Binding Factor Alpha 2 Subunit
• RUNX1 protein, human

Topics

• Adult
• Antineoplastic Agents (therapeutic use)
• Antineoplastic Combined Chemotherapy Protocols (adverse effects)
• Chromosomes, Human, Pair 1
• Chromosomes, Human, Pair 21
• Core Binding Factor Alpha 2 Subunit (genetics)
• Fatal Outcome
• Flow Cytometry
• Gene Rearrangement
• Humans
• Immunophenotyping
• In Situ Hybridization, Fluorescence
• Karyotyping
• Leukemia, Myeloid, Acute (chemically induced, diagnosis, genetics)
• Male
• Neoplasms, Second Primary (chemically induced, diagnosis, genetics)