Abstract | BACKGROUND: METHODS: In this prospective study, 54 biopsies taken from suspected HD patients (five mucosal specimens and 49 full thickness specimens) were studied. In the laboratory, after preparing sections of paraffin embedded tissues, H and E staining slides were compared with immunohistochemical (IHC) staining including: S100, NSE, CD117, CD56, Cathepsin D, Vimentin, BCL2, GFAP, Synaptophysin and chromogranin. RESULTS: The study revealed 30 negative (absence of ganglion cells) cases (55.5%), 17 positive cases (31.04%) and seven suspected cases (12.9%) of ganglion cells on the H and E staining. On IHC staining with CD56 and Cathepsin D, all of the 17 positive cases detected through H and E, were confirmed for having ganglion cells and out of 30 cases reported negative on H and E staining, 28(93.3%) were reported negative and two (6.7%) positive by IHC staining. Of the seven suspected cases H and E staining), IHC staining detectedganglion cells only in five slides; two remained negative. CONCLUSIONS: IHC staining using CD56 and Cathepsin D improved the accuracy of diagnosis in HD when used in addition to H and E staining technique, especially for negative or suspicious slides.
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Authors | Mehrdad Memarzadeh, Ardeshir Talebi, Masod Edalaty, Mehrdad Hosseinpour, Nasrin Vahidi |
Journal | Journal of Indian Association of Pediatric Surgeons
(J Indian Assoc Pediatr Surg)
Vol. 14
Issue 2
Pg. 59-62
(Apr 2009)
ISSN: 1998-3891 [Electronic] India |
PMID | 20671847
(Publication Type: Journal Article)
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