Anti-obesity effect of Morus bombycis root extract: anti-lipase activity and lipolytic effect.

Abstract

AIM OF THE STUDY:

This study evaluated anti-obesity effect of the ethanolic extract of Morus bombycis root on lipase activity and lipolysis in adipocytes and adipose tissues.

MATERIALS AND METHODS:

Lipase (triacylgycerol acylhydrolase, EC 3.1.1.3) activity was determined by measuring the hydrolysis of p-nitrophenyl butyrate to p-nitrophenol at 405 nm. Lipolytic effects were assayed in fully differentiated 3T3-L1 adipocytes and adipose tissues. In vitro, phosphodiesterase (PDE) activity was also measured.

RESULTS:

Morus bombycis root extract exhibited strong anti-lipase activity, with an IC50 value of 2.07 microg/mL.In differentiated adipocytes and adipose tissues, the extract increased lipolytic effects such as decreased intracellular triglyceride and the release of glycerol. Further, the extract inhibited PDE activity in a dose-dependent manner.

CONCLUSION:

The present study suggests that Morus bombycis root extract might be of therapeutic interest with respect to the treatment of obesity.

• Authors: Young Sook Kim, Yun Mi Lee, Hyojun Kim, Junghyun Kim, Dae Sik Jang, Joo Hwan Kim, Jin Sook Kim
• Journal: Journal of ethnopharmacology (J Ethnopharmacol) Vol. 130 Issue 3 Pg. 621-4 (Aug 09 2010) ISSN: 1872-7573 [Electronic] Ireland
• PMID: 20669373 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Crown Copyright 2010 Published by Elsevier Ireland Ltd. All rights reserved.

Chemical References

• Anti-Obesity Agents
• Phosphodiesterase Inhibitors
• Plant Extracts
• Triglycerides
• Phosphoric Diester Hydrolases
• Glycerol

Topics

• 3T3-L1 Cells
• Adipocytes (drug effects, metabolism)
• Adipose Tissue (drug effects, metabolism)
• Animals
• Anti-Obesity Agents (administration & dosage, isolation & purification, pharmacology)
• Dose-Response Relationship, Drug
• Glycerol (metabolism)
• Inhibitory Concentration 50
• Male
• Mice
• Morus (chemistry)
• Obesity (drug therapy)
• Phosphodiesterase Inhibitors (administration & dosage, isolation & purification, pharmacology)
• Phosphoric Diester Hydrolases (drug effects, metabolism)
• Plant Extracts (administration & dosage, pharmacology)
• Plant Roots
• Rats
• Rats, Wistar
• Triglycerides (metabolism)