Abstract | BACKGROUND:
Craniopharyngioma accounts for 5-10% of childhood tumors and, despite of the benign histological features, its clinical course can be malignant because of critical anatomical relationships with neural and vascular structures and the possible morbidity associated to resection. Only a few studies have addressed the molecular characterization of the cyst fluid so far and the mechanisms of action of intracystic agents are not clearly understood yet. METHODS: The acidic soluble proteins contained in the cystic fluid of six patients with cystic craniopharyngioma, three of them treated with intratumoral interferon-α, were analyzed. A high performance liquid chromatography electrospray ionization mass spectrometry analysis was performed. FINDINGS: The antimicrobial peptides α- defensins 1-3 relevant for innate immunity were detected in the cystic fluid before the intratumoral treatment. Amount of peptides significantly decreased in cystic fluid during pharmacological treatment. INTERPRETATION: Detection of α- defensins 1-3 excludes that cyst fluid formation can derive from disruption of blood-brain barrier and suggests the involvement of innate immune response in pathology of craniopharyngioma cyst formation. The reduction of α- defensins could derive both from direct antitumoral effect of interferon-α on squamous epithelial cells of craniopharyngioma cyst and from its immuno-modulatory effects on the recruitment of cells of innate immune systems. Interestingly, the clinical patient outcome well correlates with the gradual reduction of α- defensins 1-3 amount. Additional studies will be necessary to establish the role of these molecules in the pathogenesis of craniopharyngioma, and further investigations will be necessary to confirm the efficacy of the antitumoral activity of interferon-α.
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Authors | Benedetta Ludovica Pettorini, Rosanna Inzitari, Luca Massimi, Gianpiero Tamburrini, Massimo Caldarelli, Chiara Fanali, Tiziana Cabras, Irene Messana, Massimo Castagnola, Concezio Di Rocco |
Journal | Child's nervous system : ChNS : official journal of the International Society for Pediatric Neurosurgery
(Childs Nerv Syst)
Vol. 26
Issue 12
Pg. 1779-84
(Dec 2010)
ISSN: 1433-0350 [Electronic] Germany |
PMID | 20668862
(Publication Type: Journal Article)
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Chemical References |
- Immunologic Factors
- Interferon-alpha
- alpha-Defensins
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Topics |
- Child
- Child, Preschool
- Chromatography, High Pressure Liquid
- Craniopharyngioma
(drug therapy, immunology, pathology)
- Cyst Fluid
(chemistry, immunology)
- Cysts
(drug therapy, immunology, pathology)
- Female
- Humans
- Immunity, Innate
(immunology)
- Immunologic Factors
(administration & dosage)
- Inflammation
(drug therapy, immunology, pathology)
- Injections, Intraventricular
- Interferon-alpha
(administration & dosage)
- Male
- Pituitary Neoplasms
(drug therapy, immunology, pathology)
- Spectrometry, Mass, Electrospray Ionization
- alpha-Defensins
(analysis, immunology, metabolism)
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