Abstract |
S-phase kinase-associated protein 2 (Skp2) is related to cellular proliferation and differentiation in laryngeal carcinoma. This study aimed to investigate the effect of Skp-2 suppression on p27 expression, cellular proliferation, apoptosis, and tumor growth in laryngeal squamous cell carcinoma. Skp2 was stably suppressed in Hep2 laryngeal carcinoma cells by lentivirus siRNA technology and Hep2-siRNA was then inoculated into nude mice. The siRNA-induced suppression of Skp2 increased p27 expression, decreased cellular proliferation, and increased apoptosis in human laryngeal carcinoma cells and tumors, indicating that Skp2 is a viable target in the gene therapy treatment of human laryngeal carcinoma.
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Authors | Jiangtao Liu, Lijun Tan, Huijun Li, Qiwei Wang, Wenyue Ji |
Journal | ORL; journal for oto-rhino-laryngology and its related specialties
(ORL J Otorhinolaryngol Relat Spec)
Vol. 72
Issue 4
Pg. 205-14
( 2010)
ISSN: 1423-0275 [Electronic] Switzerland |
PMID | 20668394
(Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright 2010 S. Karger AG, Basel. |
Chemical References |
- Proliferating Cell Nuclear Antigen
- RNA, Small Interfering
- S-Phase Kinase-Associated Proteins
- p27 antigen
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Topics |
- Animals
- Apoptosis
(physiology)
- Carcinoma, Squamous Cell
(enzymology, pathology)
- Cell Proliferation
- Disease Models, Animal
- Female
- Gene Expression Regulation, Neoplastic
- Humans
- Immunohistochemistry
- Laryngeal Neoplasms
(enzymology, pathology)
- Mice
- Polymerase Chain Reaction
- Proliferating Cell Nuclear Antigen
(biosynthesis, genetics)
- RNA, Small Interfering
(genetics)
- S-Phase Kinase-Associated Proteins
(genetics, metabolism)
- Tumor Cells, Cultured
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