C. trachomatis
antigen in first-voided urine sediments was detected by a new EIA kit using a
monoclonal antibody, IDEIA CHLAMYDIA (IDEIA, Novo Nordisk), in males with
urethritis and females with
cervicitis. The result was compared with that by Chlamydiazyme (Abbott). 1. C. trachomatis
antigen detection in male
urethritis (285 cases) by the IDEIA test: The
antigen detection rate was 37.9% (108/285) in urethral smears, and 33.7% (96/285) in first-voided urine sediments of the patients. The positive co-incidence rate between urethral smears and first-voided urine sediments was 82.4% (98/108). Thus, the detection of the
antigen seems feasible in first-voided urine sediments. 2. Comparison of C. trachomatis
antigen detection by the IDEIA and Chlamydiazyme tests: In 78 male cases with
urethritis undergoing both tests, the rates of
antigen detection from urethral smears and first-voided urine sediments were studied. The detection rate from urethral smears was 41.0% (32/78) for IDEIA, and 37.2% (29/78) for Chlamydiazyme. In first-voided urine sediments, the rate was 35.9% (28/78) for IDEIA and 24.4% (19/78) for Chlamydiazyme. In both specimens, the detection sensitivity was higher for IDEIA. 3. C. trachomatis
antigen detection in chlamydial
cervicitis (28 cases) by the IDEIA test: The
antigen detection rate was 46.4% (13/28) in urethral smears and 60.7% (17/28) in first-voided urine sediments. The detection rate in first voided urine sediments was higher. Thus, in patients suspected of having chlamydial
cervicitis, it seems necessary not only to search the
antigen in cervical smears but also to study the first-voided urine sediments.(ABSTRACT TRUNCATED AT 250 WORDS)