Association analysis of the poliovirus receptor related-2 gene in patients with nonsyndromic cleft lip with or without cleft palate.

Abstract

Nonsyndromic cleft lip with or without cleft palate (nsCL/P) has a complex etiology, which involves both genetic and environmental factors. In this study, we carried out mutation screening of the poliovirus receptor related-2 (PRR2) gene, located at an orofacial cleft (OFC) linkage region 19q13 (OFC3). PRR2 Sau96I (A/G) genotypes of 212 patients with nsCLP and 221 controls were detected using a polymerase chain reaction-restriction fragment length polymerase assay. The results showed significant differences in the genotype and allele distribution of the PRR2 Sau96I (A/G) between the cases and controls. The GG genotype resulted in a significantly raised odds ratio (OR) compared with the AA genotype (OR=3.031; 95% confidence interval: 1.601, 5.742). The G allele showed a significant elevated risk (χ(2) = 26.991, p=0.000, OR=2.147; 95% confidence interval: 1.605, 2.871) compared with the A allele. Hence, our results support the hypothesis that this polymorphism contributes to the risk of nsCL/P, suggesting a possible etiologic role of PRR2 in nsCL/P.

• Authors: Huang Chengle, Ding Kaihong, Ban Fuzhi
• Journal: DNA and cell biology (DNA Cell Biol) Vol. 29 Issue 11 Pg. 681-5 (Nov 2010) ISSN: 1557-7430 [Electronic] United States
• PMID: 20662561 (Publication Type: Journal Article)

Chemical References

• Cell Adhesion Molecules
• Nectins

Topics

• Adolescent
• Base Sequence
• Cell Adhesion Molecules (genetics)
• Child
• Child, Preschool
• Chromosomes, Human, Pair 19 (genetics)
• Cleft Lip (complications, genetics)
• Cleft Palate (complications, genetics)
• Female
• Gene Frequency
• Genetic Association Studies
• Genetic Predisposition to Disease (genetics)
• Genotype
• Humans
• Infant
• Male
• Nectins
• Odds Ratio
• Polymorphism, Single Nucleotide