Abstract |
An ELISA system for measuring antibodies to nonstructural protein 1 (NS1) of Japanese encephalitis virus has already been established. This system uses an ELISA diluent containing casein, instead of bovine serum albumin (BSA). During a survey, we found that 21 (21%) of 102 children aged 1-5 years, who had no history of Japanese encephalitis vaccination and were without detectable neutralizing antibodies, showed positive results with this ELISA system. Western blotting analysis showed that sera from 19 (91%) of these 21 subjects had antibodies to BSA, but not NS1. These sera reacted with BSA antigen remaining in immunoaffinity-purified NS1 antigen. One solution to this problem was to reduce the BSA level to < or =1% of the NS1 amount. Another was to use a control well sensitized with BSA with the same amount as that contained in the NS1 antigen preparation.
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Authors | Eiji Konishi, Yoko Kitai, Koichi Nishimura, Seiya Harada |
Journal | Japanese journal of infectious diseases
(Jpn J Infect Dis)
Vol. 63
Issue 4
Pg. 296-8
(Jul 2010)
ISSN: 1884-2836 [Electronic] Japan |
PMID | 20657075
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antibodies, Viral
- Caseins
- Serum Albumin, Bovine
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Topics |
- Antibodies, Viral
(blood)
- Caseins
(immunology)
- Child, Preschool
- Encephalitis Virus, Japanese
(immunology)
- Encephalitis, Japanese
(diagnosis)
- Enzyme-Linked Immunosorbent Assay
(methods)
- False Positive Reactions
- Female
- Humans
- Infant
- Serum Albumin, Bovine
(immunology)
- Virology
(methods)
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