The analgesic and anti-inflammatory effect of WIN-34B, a new herbal formula for osteoarthritis composed of Lonicera japonica Thunb and Anemarrhena asphodeloides BUNGE in vivo.

Abstract

ETHNOPHARMACOLOGICAL RELEVANCE:

Lonicera japonica Thunb and Anemarrhena asphodeloides BUNGE have been used for the treatment of a variety of inflammatory diseases, cold and infective diseases in many countries, including Korea and China.

AIM OF THE STUDY:

This study aimed to assess the anti-nociceptive and anti-inflammatory activities of n-butanol fraction (WIN-34B) prepared from dried flowers of Lonicera japonica and dried roots of Anemarrhena asphodeloides as potential novel treatment of osteoarthritis.

MATERIALS AND METHODS:

Anti-nociceptive activities of WIN-34B (100, 200 and 400 mg/kg, p.o.) were measured using acetic acid-induced writhing response, formalin-induced paw licking, hot plate, radiant heat tail-flick, carrageenan-induced paw pressure, and Hargreaves tests, respectively. Anti-inflammatory activities of WIN-34B (100, 200 and 400 mg/kg, p.o.) were assessed using acetic acid-induced vascular permeability, carrageenan-induced paw edema, and croton oil-induced ear edema. Anti-osteoarthritis effect of WIN-34B was analyzed using monosodium iodoacetate (MIA)-induced osteoarthritis animal model.

RESULTS:

WIN-34B exhibited better anti-inflammatory activity than that of celecoxib in carrageenan at the dose of 200 mg/kg and croton oil-induced paw edema and ear edema at the doses of 200 and 400 mg/kg. WIN-34B exhibited significant anti-inflammatory effects on vascular permeability. WIN-34B also exhibited significant anti-nociceptive activities in the late phase of formalin-induced paw licking and writhing response model in mice. In radiant heat tail-flick and carrageenan-induced paw pressure tests, WIN-34B at the dose of 400 mg/kg and at the doses of 200 and 400 mg/kg presented similar activities to indomethacin and celecoxib. Compared to indomethacin WIN-34B at 400mg/kg showed similar or better anti-nociceptive activities after 1 and 2h of theraphy in the hot plate test and better anti-nociceptive activity than that of celecoxib in Hargreves test. In the MIA-induced osteoarthritis animal models, WIN-34B at 400 mg/kg exhibited similar or better anti-nociceptive property than that of celecoxib throughout the pain measurement periods.

CONCLUSION:

When compared to celecoxib, WIN-34B exhibited similar or better anti-nociceptive and anti-inflammatory activities in osteoarthritic animal models, which may become a potential novel treatment for osteoarthritis.

• Authors: Moonkyu Kang, Inho Jung, Jonghyun Hur, Soon Han Kim, Jeong Hun Lee, Ji-Yun Kang, Kyoung Chul Jung, Kyoung Soo Kim, Myung Chul Yoo, Dong-Suk Park, Jae-Dong Lee, Yong-Baik Cho
• Journal: Journal of ethnopharmacology (J Ethnopharmacol) Vol. 131 Issue 2 Pg. 485-96 (Sep 15 2010) ISSN: 1872-7573 [Electronic] Ireland
• PMID: 20643199 (Publication Type: Journal Article)
• Copyright: Copyright 2010 Elsevier Ireland Ltd. All rights reserved.

Chemical References

• Analgesics
• Anti-Inflammatory Agents
• Iodoacetates
• Plant Extracts
• Pyrazoles
• Sulfonamides
• WIN 34B
• Croton Oil
• Carrageenan
• Celecoxib
• Indomethacin

Topics

• Analgesics (pharmacology, therapeutic use)
• Anemarrhena
• Animals
• Anti-Inflammatory Agents (pharmacology, therapeutic use)
• Behavior, Animal (drug effects)
• Capillary Permeability (drug effects)
• Carrageenan
• Celecoxib
• Croton Oil
• Disease Models, Animal
• Edema (chemically induced, drug therapy)
• Flowers
• Hot Temperature
• Indomethacin (pharmacology, therapeutic use)
• Iodoacetates
• Lonicera
• Mice
• Mice, Inbred ICR
• Osteoarthritis (chemically induced, drug therapy)
• Pain (drug therapy, etiology)
• Pain Measurement (drug effects)
• Phytotherapy
• Plant Extracts (pharmacology, therapeutic use)
• Plant Roots
• Pyrazoles (pharmacology, therapeutic use)
• Rats
• Rats, Sprague-Dawley
• Rats, Wistar
• Sulfonamides (pharmacology, therapeutic use)