Abstract |
To establish the therapeutic relevance of new nanocarriers, rationalization of knowledge on their interactions with biological structures is essential. In the present study, we have investigated endocytosis and intracellular trafficking of lipid nanocapsules (LNCs) in rat glioma cells. Radiolabelled and fluorescent LNCs were synthesized by using a phase inversion process that follows the formation of an oil/water microemulsion containing triglycerides, lecithins and a non-ionic surfactant, the hydroxystearate of poly( ethylene glycol) (HS-PEG). Our data revealed that LNCs were rapidly accumulated within cells (from 2 min exposure) through active and saturating mechanisms involving endogenous cholesterol with a major contribution of clathrin/caveolae-independent pathways. Although initially present in endosomes, LNCs can bypass the endo-lysosomal compartment with only 10% of the cell-internalized fraction found in isolated lysosomes after 2 h exposure. As demonstrated by use of lysosomal probes, LNCs reverted lysosome integrity similarly to V- ATPase inhibitors and in a size-dependent fashion with best efficiency for small nanoparticles. When loaded with paclitaxel, smallest LNCs also triggered the best cell death activity. Those LNC properties are ascribed to the proportion of HS-PEG they provided to the cell. They are important to consider toward the development of nanomedicines that use drugs sensitive to lysosomal degradation or that need to reach extra endo-lysosomal targets.
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Authors | Archibald Paillard, François Hindré, Caroline Vignes-Colombeix, Jean-Pierre Benoit, Emmanuel Garcion |
Journal | Biomaterials
(Biomaterials)
Vol. 31
Issue 29
Pg. 7542-54
(Oct 2010)
ISSN: 1878-5905 [Electronic] Netherlands |
PMID | 20630585
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | 2010 Elsevier Ltd. All rights reserved. |
Chemical References |
- Drug Carriers
- Lecithins
- Nanocapsules
- Surface-Active Agents
- Triglycerides
- Technetium
- Cholesterol
- Paclitaxel
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Topics |
- Animals
- Cell Line, Tumor
- Cells, Cultured
- Cholesterol
(metabolism)
- Drug Carriers
(chemistry, metabolism)
- Flow Cytometry
- Lecithins
(chemistry)
- Lysosomes
(metabolism)
- Microscopy, Confocal
- Nanocapsules
(chemistry)
- Nanotechnology
- Paclitaxel
(metabolism)
- Rats
- Signal Transduction
(physiology)
- Surface-Active Agents
(chemistry)
- Technetium
(metabolism)
- Triglycerides
(chemistry)
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