[Studies on mechanism of myricetin-induced apoptosis in human hepatocellular carcinoma HepG-2 cells].

Abstract

OBJECTIVE:

To study the mechanism of myricetin inducing the HepG-2 cell line apoptosis.

METHOD:

The MTT method was employed to study myricetin pharmacodynamics in HepG-2. The light microscope and transmission was used to identify the tumor cell apoptosis in the morphology. The FCM method and the kit of caspase 3, caspase 9 were hired to detect the apoptosis rates, the content of mitochondrial membrane electric potential and the activity of caspase in cancer cells.

RESULT:

Myricetin significantly inhibits the proliferation and induces the apoptosis of HepG-2 in a dose-dependent manner, which is accompanied with G2/M and S phase arrest. In addition, myricetin also increases the activation of caspase 3,9 and results in a depolarization and delta psi m collapse in a dose-dependent manner.

CONCLUSION:

The molecular pathway of apoptosis of human hepatocellular carcinoma cell lines induced by myricetin might deal with the mitochondria-mediated pathway.

• Authors: Xiujuan Zhang, Yun Ling, Hua Yu, Yubin Ji
• Journal: Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica (Zhongguo Zhong Yao Za Zhi) Vol. 35 Issue 8 Pg. 1046-50 (Apr 2010) ISSN: 1001-5302 [Print] China
• PMID: 20617691 (Publication Type: English Abstract, Journal Article)

Chemical References

• Flavonoids
• myricetin

Topics

• Apoptosis (drug effects)
• Carcinoma, Hepatocellular (pathology)
• Cell Cycle (drug effects)
• Cell Proliferation (drug effects)
• Flavonoids (pharmacology)
• Flow Cytometry
• Hep G2 Cells
• Humans
• Membrane Potentials (drug effects)