Gentamicin-loaded wound dressing with polyvinyl alcohol/dextran hydrogel: gel characterization and in vivo healing evaluation.

Abstract

To develop a gentamicin-loaded wound dressing, cross-linked hydrogel films were prepared with polyvinyl alcohol (PVA) and dextran using the freezing-thawing method. Their gel properties such as gel fraction, swelling, water vapor transmission test, morphology, tensile strength, and thermal property were investigated. In vitro protein adsorption test, in vivo wound healing test, and histopathology were performed. Dextran decreased the gel fraction, maximum strength, and thermal stability of hydrogels. However, it increased the swelling ability, water vapor transmission rate, elasticity, porosity, and protein adsorption. The drug gave a little positive effect on the gel properties of hydrogels. The gentamicin-loaded wound dressing composed of 2.5% PVA, 1.13% dextran, and 0.1% drug was more swellable, flexible, and elastic than that with only PVA because of its cross-linking interaction with PVA. In particular, it could provide an adequate level of moisture and build up the exudates on the wound area. From the in vivo wound healing and histological results, this gentamicin-loaded wound dressing enhanced the healing effect more compared to conventional product because of the potential healing effect of gentamicin. Thus, this gentamicin-loaded wound dressing would be used as a potential wound dressing with excellent forming and improved healing effect in wound care.

• Authors: Ma-Ro Hwang, Jong Oh Kim, Jeong Hoon Lee, Yong Il Kim, Jeong Hoon Kim, Sun Woo Chang, Sung Gju Jin, Jung Ae Kim, Won Seok Lyoo, Sung Soo Han, Sae Kwang Ku, Chul Soon Yong, Han-Gon Choi
• Journal: AAPS PharmSciTech (AAPS PharmSciTech) Vol. 11 Issue 3 Pg. 1092-103 (Sep 2010) ISSN: 1530-9932 [Electronic] United States
• PMID: 20607628 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Anti-Bacterial Agents
• Dextrans
• Drug Carriers
• Gentamicins
• Hydrogels
• Polyvinyl Alcohol

Topics

• Administration, Topical
• Animals
• Anti-Bacterial Agents (administration & dosage, chemistry)
• Bandages
• Dextrans (chemistry)
• Drug Carriers (administration & dosage, chemical synthesis)
• Gentamicins (administration & dosage, chemistry)
• Hydrogels (chemistry)
• Male
• Polyvinyl Alcohol (chemistry)
• Rats
• Rats, Sprague-Dawley
• Treatment Outcome
• Wound Healing (drug effects)
• Wounds, Penetrating (drug therapy, pathology)