HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

MicroRNA profiling in human diploid fibroblasts uncovers miR-519 role in replicative senescence.

Abstract
MicroRNAs (miRNAs) are short non-coding RNAs that regulate diverse biological processes by controlling the pattern of expressed proteins. In mammalian cells, miRNAs partially complement their target sequences leading to mRNA degradation and/or decreased mRNA translation. Here, we have analyzed transcriptome-wide changes in miRNAs in senescent relative to early-passage WI-38 human diploid fibroblasts (HDFs). Among the miRNAs downregulated with senescence were members of the let-7 family, while upregulated miRNAs included miR-1204, miR-663 and miR-519. miR-519 was recently found to reduce tumor growth at least in part by lowering the abundance of the RNA-binding protein HuR. Overexpression of miR-519a in either WI-38 or human cervical carcinoma HeLa cells triggered senescence, as measured by monitoring beta-galactosidase activity and other senescence markers. These data suggest that miR-519 can suppress tumor growth by triggering senescence and that miR-519 elicits these actions by repressing HuR expression.
AuthorsBernard S Marasa, Subramanya Srikantan, Jennifer L Martindale, Mihee M Kim, Eun Kyung Lee, Myriam Gorospe, Kotb Abdelmohsen
JournalAging (Aging (Albany NY)) Vol. 2 Issue 6 Pg. 333-43 (Jun 2010) ISSN: 1945-4589 [Electronic] United States
PMID20606251 (Publication Type: Journal Article, Research Support, N.I.H., Intramural)
Chemical References
  • MIRN519 microRNA, human
  • MicroRNAs
Topics
  • Blotting, Western
  • Cell Line
  • Cellular Senescence (genetics)
  • Diploidy
  • Fibroblasts (metabolism)
  • Gene Expression Profiling
  • HeLa Cells
  • Humans
  • MicroRNAs (genetics, metabolism)
  • Reverse Transcriptase Polymerase Chain Reaction

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: