[Analysis of the circulating immune complexes in experimental lens-induced uveitis].

Abstract

The authors isolated the circulating immune complexes in experimental lens-induced uveitis by PEG-6000 precipitation and molecular filtration in two peaks. ELISA showed that Peak I was a fraction of antigens comprising four components of soluble lens crystallin, of which gamma-crystallin was the most abundant, but no soluble antigens of the retina; Peak II was the antibodies corresponding to Peak I. SDS electrophoresis demonstrated the presence of over 10 antigen-antibody complexes. The results proved that the toxic complexes of experimental lens-induced uveitis was the lens crystallin immune complexes, and further suggested that gamma-crystallin immune complexes induced experimental uveitis as well as the alpha- and beta-crystallin immune complexes.

• Authors: Q Ma
• Journal: [Zhonghua yan ke za zhi] Chinese journal of ophthalmology (Zhonghua Yan Ke Za Zhi) Vol. 27 Issue 1 Pg. 47-50 (Jan 1991) ISSN: 0412-4081 [Print] China
• PMID: 2060408 (Publication Type: English Abstract, Journal Article)

Chemical References

• Antigen-Antibody Complex
• Crystallins

Topics

• Antigen-Antibody Complex (blood)
• Crystallins (immunology)
• Electrophoresis, Polyacrylamide Gel
• Enzyme-Linked Immunosorbent Assay
• Uveitis (immunology)