Knockdown of Cyclophilin D Gene by RNAi Protects Rat from Ischemia/ Reperfusion-Induced Renal Injury.

Abstract

BACKGROUND/AIMS:

Mitochondrial permeability transition has a critical role in ischemia/reperfusion (I/R)-induced kidney injury. It is thought that mitochondrial permeability transition occurs after the opening of the permeability transition pore, a channel which putatively consists of a voltage-dependent anion channel, adenine nucleotide translocator and cyclophilin D (CypD). Much evidence shows that CypD plays an important role in I/R-induced injury.

METHODS:

To evaluate the role of CypD following I/R renal injury, we tested the hypothesis that knockdown of CypD gene by RNA interference (RNAi) protects rat from I/R-induced renal injury.

RESULTS:

Our data show that knockdown of CypD by RNAi protects normal rat kidney cell line from hypoxia-induced necrotic death. Infection of lentivirus expressing CypD RNAi sequence produces a significant reduction of CypD at both mRNA and protein levels. Both pathologic and biochemical analyses show that knockdown of CypD by RNAi protects rat kidney from I/R-induced renal injury.

CONCLUSION:

Our study provides the evidence that CypD may be a potential target for protecting I/R-induced renal injury.

• Authors: Wei Hu, Zhiqiang Chen, Zhangqun Ye, Ding Xia, Zhongyu Xia, Jiefeng Ma, Min Zhu, Gang Chen
• Journal: Kidney & blood pressure research (Kidney Blood Press Res) Vol. 33 Issue 3 Pg. 193-9 ( 2010) ISSN: 1423-0143 [Electronic] Switzerland
• PMID: 20588055 (Publication Type: Journal Article)
• Copyright: Copyright © 2010 S. Karger AG, Basel.

Chemical References

• Cyclophilin D
• Cyclophilins

Topics

• Animals
• Cell Hypoxia (genetics)
• Cell Line
• Cyclophilin D
• Cyclophilins (deficiency, genetics)
• Gene Knockdown Techniques (methods)
• Kidney (blood supply, metabolism)
• Male
• RNA Interference (physiology)
• Rats
• Rats, Sprague-Dawley
• Reperfusion Injury (genetics, metabolism, prevention & control)