Abstract | UNLABELLED: Many Human immunodeficiency virus (HIV) candidate vaccines have been tested in clinical trials, but none was sufficiently effective in the prevention of HIV infection. A HIV vaccine should induce humoral as well as cell-mediated response, the latter including the cytotoxic CD8+ T lymphocyte (CTL) response. In this study, we immunized BALB/c mice with a purified fusion peptide Gag p24-Nef and evaluated immune responses. As for the cellular responses, the adjuvanted fusion peptide induced lymphocyte proliferation, CTL response, and cytokines IFN-gamma and IL-4 in the Th1 pattern. Humoral immune response to the adjuvanted fusion peptide included an increase in IgG antibodies of more IgG2a than IgG1 subtype. These results indicate that the employed HIV-1 peptide construct can elicit both cellular and humoral immune responses in mice. Further studies aimed at memory T cells and other aspects of immune responses are needed before a comprehensive assessment of this candidate vaccine could be provided. KEYWORDS:
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Authors | M Mahdavi, M Ebtekar, K Azadmanesh, H R Khorramkhorshid, F Rahbarizadeh, M H Yazdi, R Zabihollahi, M Abolhassani, Z M Hassan |
Journal | Acta virologica
(Acta Virol)
Vol. 54
Issue 2
Pg. 131-6
( 2010)
ISSN: 0001-723X [Print] Slovakia |
PMID | 20545443
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- AIDS Vaccines
- HIV Antibodies
- HIV Core Protein p24
- Immunoglobulin G
- Recombinant Fusion Proteins
- nef Gene Products, Human Immunodeficiency Virus
- nef protein, Human immunodeficiency virus 1
- p24 protein, Human Immunodeficiency Virus Type 1
- Interleukin-4
- Interferon-gamma
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Topics |
- AIDS Vaccines
(immunology, pharmacology)
- Animals
- Female
- HIV Antibodies
(biosynthesis)
- HIV Core Protein p24
(immunology)
- HIV-1
(immunology)
- Humans
- Immunity, Cellular
- Immunity, Humoral
- Immunoglobulin G
(biosynthesis)
- Interferon-gamma
(biosynthesis)
- Interleukin-4
(biosynthesis)
- Lymphocyte Activation
- Mice
- Mice, Inbred BALB C
- Models, Animal
- Recombinant Fusion Proteins
(immunology)
- T-Lymphocytes, Cytotoxic
(immunology)
- nef Gene Products, Human Immunodeficiency Virus
(immunology)
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