HIV-1 Gag p24-Nef fusion peptide induces cellular and humoral immune response in a mouse model.

Abstract

UNLABELLED:

Many Human immunodeficiency virus (HIV) candidate vaccines have been tested in clinical trials, but none was sufficiently effective in the prevention of HIV infection. A HIV vaccine should induce humoral as well as cell-mediated response, the latter including the cytotoxic CD8+ T lymphocyte (CTL) response. In this study, we immunized BALB/c mice with a purified fusion peptide Gag p24-Nef and evaluated immune responses. As for the cellular responses, the adjuvanted fusion peptide induced lymphocyte proliferation, CTL response, and cytokines IFN-gamma and IL-4 in the Th1 pattern. Humoral immune response to the adjuvanted fusion peptide included an increase in IgG antibodies of more IgG2a than IgG1 subtype. These results indicate that the employed HIV-1 peptide construct can elicit both cellular and humoral immune responses in mice. Further studies aimed at memory T cells and other aspects of immune responses are needed before a comprehensive assessment of this candidate vaccine could be provided.

KEYWORDS:

epitopes; fusion peptide; HIV-1 p24-Nef; immune response.

• Authors: M Mahdavi, M Ebtekar, K Azadmanesh, H R Khorramkhorshid, F Rahbarizadeh, M H Yazdi, R Zabihollahi, M Abolhassani, Z M Hassan
• Journal: Acta virologica (Acta Virol) Vol. 54 Issue 2 Pg. 131-6 ( 2010) ISSN: 0001-723X [Print] Slovakia
• PMID: 20545443 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• AIDS Vaccines
• HIV Antibodies
• HIV Core Protein p24
• Immunoglobulin G
• Recombinant Fusion Proteins
• nef Gene Products, Human Immunodeficiency Virus
• nef protein, Human immunodeficiency virus 1
• p24 protein, Human Immunodeficiency Virus Type 1
• Interleukin-4
• Interferon-gamma

Topics

• AIDS Vaccines (immunology, pharmacology)
• Animals
• Female
• HIV Antibodies (biosynthesis)
• HIV Core Protein p24 (immunology)
• HIV-1 (immunology)
• Humans
• Immunity, Cellular
• Immunity, Humoral
• Immunoglobulin G (biosynthesis)
• Interferon-gamma (biosynthesis)
• Interleukin-4 (biosynthesis)
• Lymphocyte Activation
• Mice
• Mice, Inbred BALB C
• Models, Animal
• Recombinant Fusion Proteins (immunology)
• T-Lymphocytes, Cytotoxic (immunology)
• nef Gene Products, Human Immunodeficiency Virus (immunology)