Abstract |
Mutations in KCNQ2 and KCNQ3 voltage-gated potassium channels lead to neonatal epilepsy as a consequence of their key role in regulating neuronal excitability. Previous studies in the brain have focused primarily on these KCNQ family members, which contribute to M-currents and afterhyperpolarization conductances in multiple brain areas. In contrast, the function of KCNQ5 (Kv7.5), which also displays widespread expression in the brain, is entirely unknown. Here, we developed mice that carry a dominant negative mutation in the KCNQ5 pore to probe whether it has a similar function as other KCNQ channels. This mutation renders KCNQ5(dn)-containing homomeric and heteromeric channels nonfunctional. We find that Kcnq5(dn/dn) mice are viable and have normal brain morphology. Furthermore, expression and neuronal localization of KCNQ2 and KCNQ3 subunits are unchanged. However, in the CA3 area of hippocampus, a region that highly expresses KCNQ5 channels, the medium and slow afterhyperpolarization currents are significantly reduced. In contrast, neither current is affected in the CA1 area of the hippocampus, a region with low KCNQ5 expression. Our results demonstrate that KCNQ5 channels contribute to the afterhyperpolarization currents in hippocampus in a cell type-specific manner.
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Authors | Anastassios V Tzingounis, Matthias Heidenreich, Tatjana Kharkovets, Guillermo Spitzmaul, Henrik S Jensen, Roger A Nicoll, Thomas J Jentsch |
Journal | Proceedings of the National Academy of Sciences of the United States of America
(Proc Natl Acad Sci U S A)
Vol. 107
Issue 22
Pg. 10232-7
(Jun 01 2010)
ISSN: 1091-6490 [Electronic] United States |
PMID | 20534576
(Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)
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Chemical References |
- KCNQ Potassium Channels
- KCNQ2 Potassium Channel
- KCNQ3 Potassium Channel
- KCNQ5 channel, mouse
- Kcnq2 protein, mouse
- Kcnq3 protein, mouse
- Mutant Proteins
- Nerve Tissue Proteins
- DNA
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Topics |
- Amino Acid Substitution
- Animals
- Base Sequence
- DNA
(genetics)
- Female
- Gene Knock-In Techniques
- Hippocampus
(metabolism)
- In Vitro Techniques
- KCNQ Potassium Channels
(deficiency, genetics, metabolism)
- KCNQ2 Potassium Channel
(metabolism)
- KCNQ3 Potassium Channel
(metabolism)
- Membrane Potentials
- Mice
- Mice, Mutant Strains
- Mice, Transgenic
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Mutant Proteins
(genetics, metabolism)
- Nerve Tissue Proteins
(metabolism)
- Oocytes
(metabolism)
- Patch-Clamp Techniques
- Xenopus
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