Primary structure of the large (L) RNA segment of nephropathia epidemica virus strain Hällnäs B1 coding for the viral RNA polymerase.

The L RNA segment of the nephropathia epidemica virus (NEV) strain Hällnäs B1 was characterized by molecular cloning of the corresponding cDNA and subsequent determination of the DNA nucleotide sequence. The L RNA segment is 6550 nucleotides long with complementarity of 20 bases at the 3' and 5' termini. The viral messenger sense RNA contains one major open reading frame (ORF) with a coding capacity of 2156 amino acid residues encoding a protein with a calculated molecular weight of 246 kDa and an IEP of pH 7.4. Comparison of the deduced amino acid sequences from NEV hantavirus and Bunyamwera virus (BWV) L segment messenger sense RNAs, revealed a high degree of diversity (overall amino acid identity, 17%). However, three clusters of 30-40% amino acid identity were detected. One of these domains, containing an Asp-Asp motif found in many RNA polymerases, also shares amino acid sequence homology with the PB1 polymerase component of influenza type A. These results indicate that the L RNA segment of the NEV codes for the viral RNA-dependent RNA polymerase. The data presented here complete our previous studies on the characterization of the NEV genome by cDNA sequencing of the viral M and S RNA segments.
AuthorsR Stohwasser, K Raab, G Darai, E K Bautz
JournalVirology (Virology) Vol. 183 Issue 1 Pg. 386-91 (Jul 1991) ISSN: 0042-6822 [Print] UNITED STATES
PMID2053288 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • DNA, Viral
  • RNA, Viral
  • RNA Replicase
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Bunyaviridae (enzymology, genetics)
  • DNA, Viral (chemistry)
  • Molecular Sequence Data
  • Open Reading Frames
  • RNA Replicase (biosynthesis, genetics)
  • RNA, Viral (chemistry)
  • Sequence Homology, Nucleic Acid
  • Vero Cells

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