Abstract | INTRODUCTION: MATERIALS AND METHODS: Suitable breast cancer cells were transfected with either the ALCAM expression construct or anti- ALCAM transgene, to create sublines that had differential expression of ALCAM. The growth, migration and invasion of the cells were evaluated in the presence or absence of matrix proteins prepared from human bones. RESULTS: ZR-751(DeltaALCAM) ( ALCAM knockdown) and MDA-MB-231(ALCAMexp) (overexpressing ALCAM) were constructed. MDA MB-231(ALCAMexp) cells showed a slower rate of growth compared with control cells. However, in the presence of bone matrix proteins, MDA MB-231(ALCAMexp) showed a significantly reduced rate of growth, p<0.01 vs. control cells. In contrast, ZR-751(DeltaALCAM) cells grew faster compared with control cells. MDA MB-231(ALCAMexp) displayed a significantly reduced (p=0.012) and ZR-751(DeltaALCAM) cells significantly increased invasiveness (p=0.02) vs. their respective controls cells. In an ECIS-based cell migration assay, MDA-MB-231(ALCAMexp) cells showed marked reduction in migration. Inclusion of bone matrix proteins therefore further reduced the migration speed of MDA MB-231(ALCAMexp) cells. CONCLUSION:
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Authors | Simon Davies, Wen G Jiang |
Journal | Anticancer research
(Anticancer Res)
Vol. 30
Issue 4
Pg. 1163-8
(Apr 2010)
ISSN: 1791-7530 [Electronic] Greece |
PMID | 20530423
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- ALCAM protein, human
- Antigens, CD
- Cell Adhesion Molecules, Neuronal
- Fetal Proteins
- RNA, Messenger
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Topics |
- Antigens, CD
(biosynthesis, genetics)
- Bone Neoplasms
(genetics, metabolism, secondary)
- Breast Neoplasms
(genetics, metabolism, pathology)
- Cell Adhesion
(physiology)
- Cell Adhesion Molecules, Neuronal
(biosynthesis, genetics)
- Cell Growth Processes
(physiology)
- Cell Line, Tumor
- Cell Movement
(physiology)
- Extracellular Matrix
(genetics, metabolism, pathology)
- Female
- Fetal Proteins
(biosynthesis, genetics)
- Gene Expression Profiling
- Humans
- Neoplasm Invasiveness
- RNA, Messenger
(biosynthesis, genetics)
- Transfection
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