Characterization of the allergen filarial tropomyosin with an invertebrate specific monoclonal antibody.
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| Abstract |
Tropomyosins of invertebrates are pan-allergens responsible for wide spread allergic reactions against seafood and arthropods. As invertebrate tropomyosins are highly conserved, helminth tropomyosins are likely to show properties similar to these medically important allergens. Studies with a monoclonal antibody, NR1, raised against tropomyosin of the rodent filarial nematode Acanthocheilonema viteae revealed a B cell epitope common to helminths and marine mollusks, which does not occur in vertebrate tropomyosin. This antibody detected tropomyosin of A. viteae, other filariids, nematodes, trematodes and a cestode, and recognized as well tropomyosin of oyster, squid and octopus, but not of arthropods and vertebrates. Immunohistological analyses of A. viteae, Onchocerca volvulus and other nematodes using NR1 showed that tropomyosin is located in the fibrillar part of the body wall muscles and the uterus, and is also conspicuous in muscles of the pharynx, the vagina and other organs of the nematodes. The abundance of a pan-allergen like tropomyosin in parasitic worms and the counterintuitive, but well documented protection against allergic reactivity by some chronic helminth infections is discussed.
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| Authors | Michal J Sereda, Susanne Hartmann, Dietrich W Büttner, Rudolf Volkmer, Marc Hovestädt, Norbert Brattig, Richard Lucius |
| Journal | Acta tropica (Acta Trop) Vol. 116 Issue 1 Pg. 61-7 (Oct 2010) ISSN: 1873-6254 [Electronic] Netherlands |
| PMID | 20525500 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't) |
| Copyright | Copyright 2010 Elsevier B.V. All rights reserved. |
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