Abstract | OBJECTIVE: METHODS: Cardiocytes were harvested from neonatal rats and cultured. Cultured myocardial cells were divided into the control group, the hypoxia/reoxygenation group, the EPO group and the chelerythrine group, and a hypoxia/reoxygenation model of cardiocytes was reproduced. Apoptosis rate was assayed by flow cytometry. Flavoprotein fluorescence was scanned by confocal laser microscope to assess the mitoKATP channel activity. RESULTS: Apoptosis rate was significantly higher in hypoxia/reoxygenation group than that of control group [(42.56+/-8.00)% vs. (17.88+/-2.00)%, P<0.05]. There was no statistically significant difference in flavoprotein fluorescence between this group and the control group [(0.278+/-0.170)x10(-2) vs. (0.149+/-0.050)x10(-2), P>0.05]. Myocardial cell apoptosis rate in EPO group was lower than that in hypoxia/reoxygenation group [(22.73+/-5.00)% vs. (42.56+/-8.00)%, P<0.05], and flavoprotein fluorescence intensity was significantly enhanced when compared with hypoxia/reoxygenation group [(2.201+/-1.090)x10(-2) vs. (0.278+/-0.170)x10(-2), P<0.01]. However, when chelerythrine was added, the anti-apoptosis effect of EPO was blocked, and the intensity of cardiocytes flavoprotein fluorescence was decreased [the apoptosis rate was (46.72+/-17.00)% and the flavoprotein fluorescence intensity was (0.986+/-0.320)x10(-2) ]. When compared with EPO group there was statistically significant difference (P<0.01 and P<0.05). CONCLUSION: Myocardial cell apoptosis occurs in hypoxia/reoxygenation injury, and EPO can protect rat cardiomyocytes from hypoxia/reoxygenation induced apoptosis. The protective effect is partly associated with the PKC/ mitoKATP pathway.
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Authors | Hua-jun Wang, Hui-lin Jiang, Xiao-hui Chen, Pei-yi Lin, Yong-cheng Zhu, Li-li Tao |
Journal | Zhongguo wei zhong bing ji jiu yi xue = Chinese critical care medicine = Zhongguo weizhongbing jijiuyixue
(Zhongguo Wei Zhong Bing Ji Jiu Yi Xue)
Vol. 22
Issue 5
Pg. 302-5
(May 2010)
ISSN: 1003-0603 [Print] China |
PMID | 20519083
(Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Potassium Channels
- mitochondrial K(ATP) channel
- Erythropoietin
- Protein Kinase C
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Topics |
- Animals
- Apoptosis
(drug effects)
- Cell Hypoxia
(drug effects)
- Cells, Cultured
- Erythropoietin
(pharmacology)
- Myocytes, Cardiac
(drug effects, metabolism, pathology)
- Potassium Channels
(metabolism)
- Protein Kinase C
(metabolism)
- Rats
- Rats, Sprague-Dawley
- Signal Transduction
(drug effects)
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